Sequences from confirmation amplicon


Confirmation sequences for line 491A12 and hit At4g13000 are shown.
Line ID 491A12
Locus At4g13000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7598329
Primers used for PCR HF40 - 8474, show primer details
   
Sequence Name 50-K101327-0022-491-A12C-8409
GenBank Accession LN507105 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K101327-0022-491-A12C-8409
CCGGGGACATTAATCAATTGCTCTTAACGATGTCGTTTCTCCGACCAAACTCGGCGGTTC
AGTTAATATCTTTAAAAAAGTCTCTTTACGGTTGGACCCCCTAAACGGCGTCGCTCCGTA
CAACATCTCATACAAAACCACTCCCAACGACCACCAATCCACGGCGAAGTCATGGCCACT
TCCGGTGATTACCTCCGGCGCAACATATTCCTCTGTTCCAACAAACGAGTTCGACTTCTC
TCCTGAAAACTCCGACTCGGAGGATCTCGAAACCGAGTCGTCCGGGGAGATTCCGGAGTT
ACATAAACCGGAAAATGCGAAGATAGACCTTTCTTTCTTCGTCGCCGTCGAAAGTCTCGG
TGACGACGAANAAGAAGATTGTGGAGTTCTAGGAGGTAGATTCGTCGAAAGATCAAAATC
AACAAGCATCAGATGACCATTCTCTTGAATCATCACATTATCAGGCTTCAAATCTCTGTA
CACAATTCCTTGATTATGCAAATACTCAAGTGCAATCACAAGCTCCGCCGCGTAAAATCT
AATGATCTCATCGGAAAACATTTCTTCCGACTGTTTTTTCCTTAAAGAGTTGAGATCTCT
TCCAGGACAGTAATCAATCGCGTAACCGATNACTTTATCGGTGGATATAACACCATGAAG
ACGTGGAAACAGAGGATGGTCGAATCGAGACAACACTCCTTGTTCGAAACTTATGCGTTT
GTACTCA
   
Sequence Name 50-K101333-0022-491-A12C-HF40
GenBank Accession LN507106 [GenBank]
Sequencing primer gene specific primer, HF40: 5'-AAAGAAGGCCAAAGATGAGTACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K101333-0022-491-A12C-HF40
GATTCGACCATCCTCTGTTTCCCGTCTTCATGGTGTTATATCCATCGAATAAGTTATCGG
TTACGCGATTGATTACTGTCCTGGAAGAGATCTCAACTCTTTAAGGAAAAAACAGTCGGA
AGAAATGTTTTCCGATGAGATCATTAGATTTTACGCGGCGGAGCTTGTGATTGCACTTGA
GTATTTGCATAATCAAGGAATTGTGTACAGAGATTTGAAGCCTGATAATGTGATGATTCA
AGAGAATGGTCATCTGATGCTTGTTGATTTTGATCTTTCGACGAATCTACCTCCTAGAAC
TCCACAATCTTCTTTTTCGTCGTCACCGAGACTTTCGACGGCGACGAAGAAAGAAAGGTC
TATCTTCGCATTTTCCGGTTTATGTAACTCCGGAATCTCCCCGGACGACTCGGTTTCGAG
ATCCTCCGAGTCGGAGTTTTCAGGAGAGAAGTCGAACTCGTTTGTTGGAACAGAGGAATA
TGTTGCGCCGGAGGTAATCACCGGAAGTGGCCATGACTTCGCCGTGGATTGGTGGTCGTT
GGGAGTGGTTTTGTATGAGATGTTGTACGGAGCGACGCCCTTTAGGGGGTCCCACCGTAA
AGAGACTTTTTTAAAGATATTAACTGAACCGCCCAGTTTGGTCGGAGAAA

Last Updated on 06.12.2018 16:11