Sequences from confirmation amplicon


Confirmation sequences for line 491E04 and hit At4g16990 are shown.
Line ID 491E04
Locus At4g16990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9560756
Primers used for PCR JV44 - 8474, show primer details
   
Sequence Name 75-K101386-0022-491-E04C-8409
GenBank Accession LN513208 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K101386-0022-491-E04C-8409
ATTTTTCTCATTTATTTTTTCGGTCAAACGTTTTGAGAGAACTGCATAGTTAAACGTGCT
TGGGATAGATAAGTTTCAGGTGGGTAAACTTCCGATAAGTGATTGTCGGAACAGTGTGTA
TGAGGATTAAACACAGAGAAAGATCGTGTGGTGATTGTAGTGTCGGTAAACAAGTTTTTA
AAGCCTCCCAGCTGTAATGTATCAACCGTCGGAAAGGGGTGGGTCCCACGAGCCTGAGTA
AAGACGTGGGTGCTCACTAGAAAAGGCAGTCCATGCGTTATAGATAGACTCTCGTTATGC
TATTAGGGATGTTCCTTGATAACAGTTGACTATTGTTATTTTTATGTAGGCGTAGCGAAG
CAGAAATGCTTGAAAATATCGCCNAGGATGTTTCAAACAAACTCTTCCCCCCATCANATA
ATTTCAGTGACTTCGTCGGGATTGAAGCTCAAA
   
Sequence Name 75-K101392-0022-491-E04C-JV44
GenBank Accession LN513209 [GenBank]
Sequencing primer gene specific primer, JV44: 5'-CTCTATATGAGCTTCAATCCCGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K101392-0022-491-E04C-JV44
TTGATGGGGGGAGAGTTTGTTTGAACATCCTTGGCGATATTTTCAGCATTTCTGCTTCGC
TACGCCTACATAAAAATAACAATAGTCAACTGTTATCAAGGAACATCCCTAATAGCATAA
CGAGAGTCTATCTATAACGCATGGACTGCCTTTTCTAGTGAGCACCCACGTCTTTACTCA
GGCTCGTGGGACCCACCCCTTTCCGACGGTTGATACATTACAGCTGGGAGGCTTTAAAAA
CTTGTTTACCGACACTACAATCACCACACGATCTTTCTCTGTGTTTAATCCTCATACACA
CTGTTCCGACAATCACTTATCGGAAGTTTACCCACCTGAAACTTATCTATCCCAAGCACG
TTTAACTATGCAGTTCTCTCAAAACGTTTGACCGAAAAAATAAATGTAGAANAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11