Sequences from confirmation amplicon


Confirmation sequences for line 493A10 and hit At2g02810 are shown.
Line ID 493A10
Locus At2g02810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:803079
Primers used for PCR MP30 - 8474, show primer details
   
Sequence Name 59-K020436-022-493-A10C-8409
GenBank Accession LN509865 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K020436-022-493-A10C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAATATGATGGTCGTGGGACATGAAGATGAA
GTAATGTCCCACGGCACCGCATATACAATACTTTAGAATGTCCCATGCCGCTTCCGGGTG
TAGCTTACAGAACTGAATTGCTTTGAATCCTATCCCTTGTGGCAAGCCAAACATGTAGAT
CATGTTGTATATTGTGCCCCATAAGTTCATTCCCAGCATTATGTCCCACGCTTCGGTTTT
CGGGTACCTATAAAAATTCATTGTGTGGTGACTTGATAAAAGATGGAAGATTGTAAGTTC
TATGTTCTGTTGCAATGTCAGATCAATATATACCTTGAGGCAATGGAGTCTTGTGTGGCA
TTTGTGAATCCGTCAAAGGCGAGGTTTAAGGAACAAAGTGCGTAACCGAGGGGAGCATTT
GGATGTGCTAGCTTGCTAATTGTCTTAGAGCTTGTCTGAAAAAAATAAAAAAAATCAAAA
GATCAATAATGTGCTGTGATCAAAGTTTTTGGATGAGTAGATTTACATACCTTAAGAAGA
GCAAAGATGGATACTCCTCCAGCGACAAGAAAGGTGCACATGTATTCAGGGAAAGTG
   
Sequence Name 60-K020436-022-493-A10C-MP30
GenBank Accession LN510133 [GenBank]
Sequencing primer gene specific primer, MP30: 5'-ACGGAATAAGATACACTTTCCCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K020436-022-493-A10C-MP30
CATGTGCACCTTTCTTGTCGCTGGAGGAGTATCCATCTTTGCTCTTCTTAAGGTATGTAA
ATCTACTCATCCAAAAACTTTGATCACAGCACATTATTGATCTTTTGATTTTTTTTATTT
TTTTCAGACAAGCTCTAAGACAATTAGCAAGCTAGCACATCCAAATGCTCCCCTCGGTTA
CGCACTTTGTTCCTTAAACCTCGCCTTTGACGGATTCACAAATGCCACACAAGACTCCAT
TGCCTCAAGGTATATATTGATCTGACATTGCAACAGAACATAGAACTTACAATCTTCCAT
CTTTTATCAAGTCACCACACAATGAATTTTTATAGGTACCCGAAAACCGAAGCGTGGGAC
ATAATGCTGGGAATGAACTTATGGGGCACAATATACAACATGATCTACATGTTTGGCTTG
CCACAAGGGATAGGATTCAAAGCAATTCAGTTCTGTAAGCTACACCCGGAAGCGGCATGG
GACATTCTAAAGTATTGTATATGCGGTGCCGTGGGACATTACTTCATCTTCATGTCCCAC
GACCATCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGCTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA

Last Updated on 06.12.2018 16:11