Sequences from confirmation amplicon


Confirmation sequences for line 493B02 and hit At4g16420 are shown.
Line ID 493B02
Locus At4g16420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9265970
Primers used for PCR BD65 - 8474, show primer details
   
Sequence Name 15-K028344-022-493-B02C-8409
GenBank Accession LN492832 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K028344-022-493-B02C-8409
ATATTAGTAGAAGATCCAAAACCTCACAAATTTCGACCGAGNTTTTGTTGAATTCCGATT
AGATCTTTCTTCCACGAAACCCCCCCAAGTACCCATTCCATCTTCTCCGGTGAGTCTCCC
ATGTTGGCATTACGCTAGGGTTCTTCTTCTCTGTGGTTTCGCATAACCCAATAATCCAAT
TTTCTACTTGGGCATCAATGGGTCGCTCTCGAGGGAACTTCCAAAATTTCGAAGACCCTA
CTCAGAGGTTTGTTCCTAATTCACTACTCTAATTTCCGATTTGGGGTTTTTGTGGATTTC
TCTTTTGTAATTGCCAATTTGTGCTGAAATTGATTTGAATTTCAAGTTTCTTTGAGTCTG
TGTTGTGCAATTGTCATTGATCTCTAGTTGGGACAATGAGCATGGCTTCAAATTTGTGGC
TTTTGATAACTTTCAGTAGCTATAGTCTCCTCTATCTTCATCTATGTAATTGTGCCAATT
TCTGCTGAAATTGATTGATTTTGAAGTTTCTTTGCATCTGCTTTGTGCAACAATCATTGA
TTTTTCAGTTGGGACAATGAGCATCGCTA
   
Sequence Name 15-K028344-022-493-B02C-BD65
GenBank Accession LN492833 [GenBank]
Sequencing primer gene specific primer, BD65: 5'-AACAAACTTTAAGCGATGCTCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K028344-022-493-B02C-BD65
AATCAATTTTCAGCAGAAAATTCGGCACAATTACATAGAAGAAGAATAGAGGTGAACTAT
AGCTACTGAAAGTTATCAAAAGCCACAAATTTGAAGCCATGCTCATCGTCCCAACTAGAG
ATCAATGACAATTGCACAACACAGACTCAAAGAAACTTGAAATTCAAATCAATTTCAGCA
CAAATTGGCAATTACAAAAGAGAAATCCACAAAAACCCCAAATCGGAAATTAGAGTAGTG
AATTAGGAACAAACCTCTGAGTAGGGTCTTCGAAATTTTGGAAGTTCCCTCGAGAGCGAC
CCATTGATGCCCAAGTAGAAAATTGGATTATTGGGTTATGCGAAACCACAGAGAAGAAGA
ACCCTAGCGTAATGCCAACATGGGAGACTCACCGGAGAAGATGGAATGGGTACTTGGGGG
GGTTTCGTGGAAGAAAGATCTAATCGGAATTCAACAAAACTCGGTCGAAATTTGTGAGGT
TTTGGATCTCTACTAATATTATTCAGAAGTTCCGACCGAGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATTTCGACGTGT

Last Updated on 06.12.2018 16:11