Sequences from confirmation amplicon


Confirmation sequences for line 493E02 and hit At4g27060 are shown.
Line ID 493E02
Locus At4g27060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13582595
Primers used for PCR BD81 - 8474, show primer details
   
Sequence Name 16-K028344-022-493-E02C-8409
GenBank Accession LN493300 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K028344-022-493-E02C-8409
CTCTTGCCATGTCTTCAACAATCCTTTCTAAACCCCTTACTCTGCCCTCCAAGGTCACCA
TACTGTCATGTGAGCCACCAATAAACTCTTGGAGCATGTTCATAAGATTTGTTTGTTGTC
TCTCTAGCTGCAACAACTGCCTCTGGATGGCTGACCAATTTCCTCTATTACTTGTGAAGG
ATCCTTCAGATTGGTTATCAGTATTGGAGACAGGAGCGTGATGTCCAGGTGTCTCTTTTC
CGTTTACAACACCAGCCTTATCATCACCAGATGTCCTTGCTTGTGATCCGTTCCCATCAA
ACCTACCTTTTACCTGTTTATCATCTGCTTGGGTGTTCTTGAGGCGATTTGAAGATCCCA
TGGCATTGAGATCATCGAGTCCTGATTCTTCCTCATCATTGTTTTTACATCTACGAGGAA
CTACTACTTCCACGGATTGCCTTCTTTCTAGTCTTTGGAAAAACTCAGGATTAAAATCTT
TATCACTTAAGACAGGTGCTTTCTTCTTCAACAGTCCTACTGCTTTTTCAGGAAAACATC
CTTTTCCTTTA
   
Sequence Name 16-K028344-022-493-E02C-BD81
GenBank Accession LN493301 [GenBank]
Sequencing primer gene specific primer, BD81: 5'-CCAGACTCTGCTTCTAAAGGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K028344-022-493-E02C-BD81
AAAAAGAATTTAAATTCCCGAGTTTTTCCCAAGAACTAGAAAGAAGGCAATCCGTGGAAG
TAGTAGTTCCTCGTAGAAGTAAAAACAATGATGAGGAAGAATCAGGACTCGATGATCTCA
ATGCCATGGGATCTTCAAATCGCCTCAAGAACACCCAAGCAGATGATAAACAGGTAAAAG
GTAGGTTTGATGGGAACGGATCACAAGCAAGGACATCTGGTGATGATAAGGCTGGTGTTG
TAAACGGAAAAGAGACACCTGGACATCACGCTCCTGTCTCCAATACTGATAACCAATCTG
AAGGATCCTTCACAAGTAATAGAGGAAATTGGTCAGCCATCCAGAGGCAGTTGTTGCAGC
TAGAGAGACAACAAACAAATCTTATGAACATGCTCCAAGAGTTTATTGGTGGCTCACATG
ACAGTATGGTGACCTTGGAGGGCAGAGTAAGGGGTTTAGAAAGGATTGTTGAAGACATGG
CAAGAGATCTTTCCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATNTCGACGTGTCT

Last Updated on 06.12.2018 16:11