Sequences from confirmation amplicon


Confirmation sequences for line 495F02 and hit At3g54340 are shown.
Line ID 495F02
Locus At3g54340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20120020
Primers used for PCR BM92 - 8474, show primer details
   
Sequence Name 15-K104526-0022-495-F02C-BM92
GenBank Accession LN492959 [GenBank]
Sequencing primer gene specific primer, BM92: 5'-GGTAATAGTGGTGATGGTTCTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K104526-0022-495-F02C-BM92
AGAACTGAGTCGTAATCTCCTCCATTGTCTACTAGTCCATAGTGAGGATCTTCAGCTCTT
AGTTCCTAATTCACCAAAACATGTTTTAAGCCATATTCCACATAACTAAAACCAATATTA
TTAATTCGAAATGACATGACATTTGATATATGATTTAACTAGGAGATATTTCGATAAGAG
AATCACAAGAAAACATAAGAGTGACTGATCAATCTACACTACATTTCTAGATATCTTTTT
AACTGGCTCCGTATTCCATGAATGTATTATGCCTAAACCAACATTTGAGAAAAACAGAAA
GAGATATTACCAGCTCATGTATGAGATTCTTTTGTATGTCTTGTTGACTTTTGTTCTGCA
ATTGTGTAAACCACAAGAGAAATGATGAAGAAATATTCCCTCCCAAAAAAATTATGAAGA
TATATACCCAAGTTATTATGACATCACTATATAGTATACTTGGATCAATTAAATGTTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AC
   
Sequence Name 31-K104527-0022-495-F02C-8409
GenBank Accession LN500151 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K104527-0022-495-F02C-8409
ATTTACAATTGAACATTTAATTGATCCAAGTATACTATATAGTGATGTCATAATAACTTG
GGTATATATCTTCATAATTTTTTTGGGAGGGAATATTTCTTCATCATTTCTCTTGTGGTT
TACACAATTGCAGAACAAAAGTCAACAAGACATACAAAAGAATCTCATACATGAGCTGGT
AATATCTCTTTCTGTTTTTCTCAAATGTTGGTTTAGGCATAATACATTCATGGAATACGG
AGCCAGTTAAAAAGATATCTAGAAATGTAGTGTAGATTGATCAGTCACTCTTATGTTTTC
TTGTGATTCTCTTATCGAAATATCTCCTAGTTAAATCATATATCAAATGTCATGTCATTT
CGAATTAATAATATTGGTTTTAGTTATGTGGAATATGGCTTAAAACATGTTTTGGTGAAT
TAGGAACTAAGAGCTGAAGATCCTCACTATGGACTAGTAGACAATGGAGGAGATTACGAC
TCAGTTCTTGGATACCAAATCGAAGGGTCACGTGCTTACGCTCTTCGTTTCCACCAGAAC
CATCACACCTATAA

Last Updated on 06.12.2018 16:11