Sequences from confirmation amplicon


Confirmation sequences for line 500E12 and hit At5g27620 are shown.
Line ID 500E12
Locus At5g27620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9771836
Primers used for PCR CK64 - CR3S, show primer details
   
Sequence Name 61-K036418-022-500-E12C-CK64
GenBank Accession LN510449 [GenBank]
Sequencing primer gene specific primer, CK64: 5'-TCAGCTGAGAGTAACAAAGCAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K036418-022-500-E12C-CK64
GACAACAAACTATGATACGTATGAAGTAGATATTGAAGGAACCAAAATTTTTACCTTGAT
CTCCTGAACCAACTTTATCTTTAGGATATGTTAGTGATCCACTAGCATCTACTTCAACTT
GAGTTGTTCCACACTATGAATAACAATAAGTTAAAATCTAAACATGAATACAGGACTAAA
GCTCCATATCAACAATTATTCTAACAAACCTTCTCCAGCATTTGCACTGCCCTCTGGTTA
GCAGCTTTATATCTCTCTGCCTACAATTACAACATTACAGCTTAGTTGCAATACCAATAT
CTCTACACAACAAGTAAGATAATAACAACAACAACACACATAACTAAGTAACAAAGAGTT
CCAAATGCTCGATTAATCCGACTCTCTATGAAGCAATCAAAGCAAAGAAAATCCAAAGAT
GAACAAAATATAATTATAAAGCCGCATTGTAGTGATCAGAAGGAGAAGGGCTGAAAATAC
ATACTAAGGGTTTCTTATATGCTC
   
Sequence Name 53-K036418-022-500-E12C-3144
GenBank Accession LN508074 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K036418-022-500-E12C-CR3S
CTTCTGATCACTACAATGCGGCTTTATAATTATATTTTGTTCATCTTTGGATTTTCTTTG
CTTTGATTGCTTCATAGAGAGTCGGATTAATCGAGCATTTGGAACTCTTTGTTACTTAGT
TATGTGTGTTGTTGTTGTTATTATCTTACTTGTTGTGTAGAGATATTGGTATTGCAACTA
AGCTGTAATGTTGTAATTGTAGGCAGAGAGATATAAAGCTGCTAACCAGAGGGCAGTGCA
AATGCTGGAGAAGGTTTGTTAGAATAATTGTTGATATGGAGCTTTAGTCCTGTATTCATG
TTTAGATTTTAACTTATTGTTATTCATAGTGTGGAACAACTCAAGTTGAAGTAGATGCTA
GTGGATCACTAACATATCCTAAAGATAAAGTTGGTTCAGGAGATCAAGGTAAAAATTTTG
GTTCCTTCAATATCTACTTCATACGTATCATAGTTTTGTTTGTCGAAGTTTAAATAATGT
TACTTTTTGCTTTGTTACCTTCAGC
Primers used for PCR RE22 - CR3S, show primer details
   
Sequence Name 62-K036418-022-500-E12C-RE22
GenBank Accession LN510691 [GenBank]
Sequencing primer gene specific primer, RE22: 5'-CAAAGGCTTAAGCTTCTTATCAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K036418-022-500-E12C-RE22
ACATTATTTAAACTTCGACAAACAAAACTATGATACGTATGAAGTAGATATTGAATGAAC
CAAAATTTTTACCTTGATCTCCTGAACCAACTTTATCTTTAGGATATGTTAGTGATCCAC
TAGCATCTACTTCAACTTGAGTTGTTCCACACTATGAATAACAATAAGTTAAAATCTAAA
CATGAATACAGGACTAAAGCTCCATATCAACAATTATTCTAACAAACCTTCTCCAGCATT
TGCACTGCCCTCTGGTTAGCAGCTTTATATCTCTCTGCCTACAATTACAACATTACAGCT
TAGTTGCAATACCAATATCTCTACACAACAAGTAAGATAATAACAACAACAACACACATA
ACTAAGTAACAAAGAGTTCCAAATGCTCGATTAATCCGACTCTCTATGAAGCAATCAAAG
CAAAGAAAATCCAAAGATGAACAAAATATAATTATAAAGCCGCATTGTAGTGATCAGAAG
GAGAAGGGCTGAAAATACATACTAAGGGTTTCTTATATGCTC

Last Updated on 06.12.2018 16:11