Sequences from confirmation amplicon


Confirmation sequences for line 504C02 and hit At1g77300 are shown.
Line ID 504C02
Locus At1g77300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29040113
Primers used for PCR PF10 - 8474, show primer details
   
Sequence Name 52-K102872-0022-504-C02C-8409
GenBank Accession LN507778 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K102872-0022-504-C02C-8409
CATTTACATTGAATATACTCATTACTCTTTCTTTTTCTCCATATTGATCCATCATACTCT
TTCTTTTTCTCCATTTCAACTTCATCGACTGTACTTCTCTAAAATTAAGAATACTTAACT
GTTGAGCTTCTTCTCTAAAGTTAGATTTCCAGGTATAGGGCTGTTTGCTGTTTTCTCCCC
CCCATTGTTCCGCAACCATGGAGGAACACTCTGTTTCTGTTGCCGAAAGTAAGTTGTCCC
TATATCAGATGAAAACAACTCTCTCTTACGGGTTGGAGCGGTCGAGTCAGTCACAGGTGT
AGCTGGTAAGATCTCATTGGAGTAAGTCAGGTTTCCCATGGACGAGGAACAGGCTCTTAC
GTTTCTCTTAGCAAAAAACTCACCATGAGACACTGGCGGTAGAGGTGGAAATGGATAGAA
CGGCATGCCAGGAGCAACAGACCAGGTGGTCGGGTCCTCTTTGCCAGGTGAACCAAATTG
ATGAACAATGCTAAGTGGAATCCCATAGGAAACTGGTAAGCGAGAAAGGAACTTTTGTTG
CGGCTGTGCAGTAATTGCATCAGGCACATCTGTGCAGGGTGATGAAAACCCGGGTGGAAG
GTCATCTTGGACATCCTGATTTCCATTAGTTTCATCTGTTTGTTGGGACAAGATAGTCAT
GATTCTTTGTTCTTTTGTCTTAGACGGCTGGTCCCATCTGCTTTTGCGCTTGCGTCCATT
TGTCTCAGGAAGACCGGAATTAGGTTCTGAATATCCCTCAGATACAATTGCTG
   
Sequence Name 52-K102873-0022-504-C02C-PF10
GenBank Accession LN507779 [GenBank]
Sequencing primer gene specific primer, PF10: 5'-AAACAGCATCTGTATCTGAGGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K102873-0022-504-C02C-PF10
GCGCAAAGCAGATGGGACCAGCCGTCTAAGAATAAAGGCGCAGAATCATGACTATCTTGT
CCCAACAAACCCATGAAACTAATGGAAATCAGGATGTCCAAGATGACCTTCCACCCGGGT
TTTCATCACCCTGCACAGATGTGCCTGATGCAATTACTGCACAGCCGCAACAAAAGTTCC
TTTCTCGCTTACCAGTTTCCTATGGGATTCCACTTAGCATTGTTCATCAATTTGGTTCAC
CTGGCAAAGAGGACCCGACCACCTGGTCTGTTGCTCCTGGCATGCCGTTCTATCCATTTC
CACCTCTACCGCCAGTGTCTCATGGTGAGTTTTTTGCTAAGAGAAACGTAAGAGCCTGTT
CCTCGTCCATGGGAAACCTGACTTACTCCAATGAGATCTTACCAGCTACACCTGTGACTG
ACTCGACCGCTCCAACCCGTAAGAGAGAGTTGTTTTCATCTGATATAGGGACAACTTACT
TTCGGCAACAGAAACAGAGTGTTCCTCCATGGTTGCGGAACAATGGGGGGGAGAAAACAG
CAAACAGCCCTATACCTGGAAATCTAACTTTAGAGAAGAAGCTCAACAGTTAAGTATTCT
TAATTTTAGAGAAGTACAGTCGATGAAGTTGAAATGGAGAAAAAGAAAGAGTATGATGGA
TCAATATGGAGAAAAAGAAAGAGTAATGAGTATATTCAATTTGTAATGGCTTCATGTCCG
GGAAATCTTCATGGATCAGCAATGAGTATGATGGTCTATATGGA

Last Updated on 06.12.2018 16:11