Sequences from confirmation amplicon


Confirmation sequences for line 511G09 and hit F1N21 are shown.
Line ID 511G09
Locus F1N21 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25170080
Primers used for PCR AB33 - 8474, show primer details
   
Sequence Name 47-K103787-0022-511-G09C-AB33
GenBank Accession LN506182 [GenBank]
Sequencing primer gene specific primer, AB33: 5'-AACAGAAATCACTCGTCCCTTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K103787-0022-511-G09C-AB33
TACTAACTAAAATCTAAACTATAAACTTTAAATTATAAACTCCAAACTCTAAAAACTAAA
CACTAAATCCAAGAACTAAAATCTAAAACCCCAAATTCTACTCAACCCTAAATTGTTAAG
CTTGAAACCGTTATAATTATATAGTTTCCTTTATTTTGTCATGATTCAACTTATTTTTTT
TTTCATTTCAAATATCTTTTAAAACCGTCGTATTTATTTGGTTACACATTTCAAATTACT
AAAATGATAAAATACTATTTTTATGTGATAACAAAAAAAAAAACTATTTTTTCAAGGGTT
TTGAAAATAAACATTTGGGGGCAAAAAGGCAAATTTGGCATTTGCCCCCACGGAAAATAA
AAAAAACCCTGGACAACAACTTAAAAACTTCAAAAAAAAATTGAAAACAACAAATTTTTT
CAAAATCTTCATTCGAAGGGAAAACCAACTCAGGTAAAATTAAACCCCCTTTTCTTTTTT
AACAAAAACCCTAAAAAAGGGAAAAAGGTTAATTTAACTTTTTTATATGTACCCAACACA
TTTTTCCCAAATCTTTGTTTCCTTTAACCGGTAAAAACAAATTCCCCCCCCAAAAAAAAT
CTAATTTTCCTTTTCCGGGGCTCCTGGTCCGGAAAATCTCCTGGAATAACCATTAAGAAT
AAGGGTAAAAATGAAAAAAAAAAAAAATTATTTACCATTTTTTTTTCATTTCC
   
Sequence Name 63-K103788-0022-511-G09C-8409
GenBank Accession LN510976 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K103788-0022-511-G09C-8409
GGAATGGAAATTAGATTTTTTTTGGTGGGTGAATTAGTTTTGACCGGTTAACGAAACTAT
AGATATGGAATAAATGTGTAGTGTACATATAACAACGTAACATAAACATTATCCCTTTAT
TTAGGGTTTTTGTCAAAAGAGAAAAGAGGCTTTAATGTGACCTGAGTTGGTTTCCCGTAC
GATTGAAGATTGTGAAAAGATTTGTCGTTTTCAATTTTCTTTTGACGTCTCTAGCTAGTA
GTCAAGGGTTGTTCTAATTTACAGTGGGCGCAAATGCAATATATGCATTCATGCACCCAA
CTGTTTATATACAAAACCATAGAAAATATAGTTTTTTTTTTTGTTATCACATAAAAATAG
TATTTTATCATTTTAGTAATTTGAAATGTGTAACAATATAAATACAACGGTTTTAAAAAA
TATTTGAAATGAAAAAAAAAATATTTTGAATCAGGACAAAATAAGGGAAACTATTTAATT
ATAACGGTTTCAACCTAAACATTTTAGGGTTAATAAAAATTTGGGGTTTAAAATTTAATT
TCTGGAATTTAGGGTTAATTTTTAAAATTTTGAATTTTAAAATTTAAATTTAAAGGTTGA
AATTTTATTTAGTATTGTAAATTTAAACAAATCTCCCCAATTTTCACCCCAAGTAATTTA
AGGGAACAAGTGATTTCTTTTAAA
Primers used for PCR AB34 - 8474, show primer details
   
Sequence Name 65-K104197-0022-511-G09C-AB34
GenBank Accession LN511391 [GenBank]
Sequencing primer gene specific primer, AB34: 5'-GCAGATCTGTTCTTCTCCTCTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K104197-0022-511-G09C-AB34
GTTACACTTTTTTCTAGGGTTTTCATTCCAGATCTAATATCTGGTTATGAACTTATATAT
TGGGGATTAACCTCAAACTCAACTATTTTTAGTATCTTCTTAACATTTTACTTTTACTAC
GTATACATATACAAGTAGGTAACGTACCTTTCGTATGAGTGCTCCACAAAAGCAACATCT
AATTAACATGGCCCATATAGTCCAAAAATAATAATTCAACAATAGAAGACGACATATGAA
GGATTTGTTTCCATTTCAATGATAAACATCACGCTTTTAAGAGTCAGTATTGAAGGCGAT
CGATGTTCTTGAATGTATAGTATTTCACTGTTTTTATTTTTTGTCGGATCTCAGACAAGA
TCTGCTTTGCTTTCGTTTTGGATATAAAAAAAAACTTCTATTCTTTTGTCCAGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCACGNTTATT
   
Sequence Name 75-K104198-0022-511-G09C-8409
GenBank Accession LN513235 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K104198-0022-511-G09C-8409
TTACATTGAAATATCCTGGACAAAAGAATAGAAGTTTTTTTTTATATCCAAAACGAAAGC
AAAGCAGATCTTGTCTGAGATCCGACAAAAAATAAAAACAGTGAAATACTATACATTCAA
GAACATCGATCGCCTTCAATACTGACTCTTAAAAGCGTGATGTTTATCATTGAAATGGAA
ACAAATCCTTCATATGTCGTCTTCTATTGTTGAATTATTATTTTTGGACTATATGGGCCA
TGTTAATTAGATGTTGCTTTTGTGGAGCACTCATACGAAAGGTACGTTACCTACTTGTAT
ATGTATACGTAGTAAAAGTAAAATGTTAAGAAGATACTAAAAATAGTTGAGTTTGAGGTT
AATCCCCAATATATAAGTTCATAACCAGATATTAGATCTGGAATTGAAAACCCTAGAAAA
AAGTTGTAAACCCTAGTGAAAAATTAACCTGTAACTGAGTGAGAGAGGAGAAGAACAGAT
CTG

Last Updated on 06.12.2018 16:11