Sequences from confirmation amplicon


Confirmation sequences for line 519C02 and hit At1g66560 are shown.
Line ID 519C02
Locus At1g66560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24833571
Primers used for PCR IS49 - 8474, show primer details
   
Sequence Name 35-K022408-022-519-C02C-8409
GenBank Accession LN501662 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K022408-022-519-C02C-8409
AGATTTCCCGGCACATGAAGCCATTTACAAAAAAATGTTCTCCAACATCGATCAAACAGC
TGTGGCAGCACTCCTCCGTGGCCAAGGATGCGCTAACAGTCTCAAGAGGCTCCTCGAAAA
CCACAAGTTAAGCTCAGATTCAACAGAGCCGCTCATCTACACCATACTCAATTCTTTCTC
ACTTGCTCTCTCTTTTGTGGACCCGCCTAGTCTTCTACCACACAATGAGTCTTCTCTTCA
AAACATGACAAGTCATGTTCTTCAGAGATCATCAAAGAAGTAAGTTACCAACAAAAAGCT
AAACACTCATTGCATATGTTACGAACAAAGTTCATGAGTTTCTAGCTAACTCAAAATAAA
GTTAAATAGCAATTGTTTTTTCATGTTACTTTTATGTCTCGTATAATTTTTGATATGCAA
TTATGTCTTCTATATTTTTAAGCATTTCTCCAATACATCATTGTAGAAAATATTATGGAG
CAGAAGATTTAGAGTATTACAGAGACGAATCCCCGACTCCACGCCCCGATGACGGCTTCA
CCTGGAGGAAATATGGACAAAAAACTATCAAAACCTCACCGTACC
   
Sequence Name 36-K022408-022-519-C02C-IS49
GenBank Accession LN502060 [GenBank]
Sequencing primer gene specific primer, IS49: 5'-AATTGACTAACCTTTGGTACGGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K022408-022-519-C02C-IS49
GTTTTGATAGTTTTTTGTCCATATTTCCTCCAGGTGAAGCCGTCATCGGGGCGTGGAGTC
GGGGATTCGTCTCTGTAATACTCTAAATCTTCTGCTCCATAATATTTTCTACAATGATGT
ATTGGAGAAATGCTTAAAAATATAGAAGACATAATTGCATATCAAAAATTATACGAGACA
TAAAAGTAACATGAAAAAACAATTGCTATTTAACTTTATTTTGAGTTAGCTAGAAACTCA
TGAACTTTGTTCGTAACATATGCAATGAGTGTTTAGCTTTTTGTTGGTAACTTACTTCTT
TGATGATCTCTGAAGAACATGACTTGTCATGTTTTGAAGAGAAGACTCATTGTGTGGTAG
AAGACTAGGCGGGTCCACAAAAGAGAGAGCAAGTGAGAAAGAATTGAGTATGGTGTAGAT
GAGCGGCTCTGTTGAATCTGAGCTTAACTTGTGGTTTTCGAGGAGCCTCTTGAGACTGTT
AGCGCATCCTTGGCCACGGAGGAGTGCTGCCACAGCTGTTTGATCGATGTTGGAGAACAT
TTTTTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGC

Last Updated on 06.12.2018 16:11