Sequences from confirmation amplicon


Confirmation sequences for line 519H04 and hit At4g02200 are shown.
Line ID 519H04
Locus At4g02200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:972703
Primers used for PCR HM96 - 8474, show primer details
   
Sequence Name 49-K101220-0022-519-H04C-8409
GenBank Accession LN506770 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K101220-0022-519-H04C-8409
CGGAGGAGAAATGCTCCTTTAATTATTATCTGAGATTTTTCATTCAAGTTCGTGTGGATT
CAGAATCAAAATGGAAGAAGATTTGTTGGGCATTTGTGGGTTTGATTCGTCGAAGAAATA
TCGATTAGAAGAACTTGCCAAGTATCAGTCGGGTTAGTGTAAAGATCCATTCTTTTTCCC
AATTGCATTTAGCTTTGTTTTAGGGTTTATGCTTTATATGCAGGTGGTTTTAATTTCTTT
ATCAAATTTCAATTCTTTTGGCCCTTAAGGTCTAGTGAGTTTAAATTTTGTCACTTTATG
GGTAAACTTTATGTTATGTAATTGATAAAAGTTTTGTGATGGTGAAATTTTTGTTTAGGT
TCATGTATTGAGTTTGAAGATGATGATGAAATGGCAGTGGATTATCCATGCCCGTTTTGC
TCAGATGATTATGATTTAGTTGAATTGTGTCACCATATCGATGAGGAGCATCAACTAGAC
GCCAACAATGGGGTAATTGCAACTCGTATCCATCTCCCAAATCAGTTACTGTTCTTTAAA
CTATACATTTGAGTTTTTACATGAAGCAGTTAGTTGTAAATTTAGACTATTTATCGTGTG
ATGTTCATTGTTGTACTCTAATGGTTACTTTAGTAGGCAGGATTGTGATTCTGTAAGGA
   
Sequence Name 49-K101227-0022-519-H04C-HM96
GenBank Accession LN506771 [GenBank]
Sequencing primer gene specific primer, HM96: 5'-TCCCTACCAACTCTCCTTACAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K101227-0022-519-H04C-HM96
ACATTAGAGTAACAATGAACATCACACGATAAATAGTCTAAATTTACAACTAACTGCTTC
ATGTAAAAACTCAAATGTATAGTTTAAAGAACAGTAACTGATTTGGGAGATGGATACGAG
TTGCAATTACCCCATTGTTGGCGTCTAGTTGATGCTCCTCATCGATATGGTGACACAATT
CAACTAAATCATAATCATCTGAGCAAAACGGGCATGGATAATCCACTGCCATTTCATCAT
CATCTTCAAACTCAATACATGAACCTAAACAAAAATTTCACCATCACAAAACTTTTATCA
ATTACATAACATAAAGTTTACCCATAAAGTGACAAAATTTAAACTCACTAGACCTTAAGG
GCCAAAAGAATTGAAATTTGATAAAGAAATTAAAACCACCTGCATATAAAGCATAAACCC
TAAAACAAAGCTAAATGCAATTGGGAAAAAGAATGGATCTTTACACTAACCCGACTGATA
CTTGGCAAGTTCTTCTAATCGATATTTCTTCGACGAATCAAACCCACAAATGCCCAACAA
ATCTTCTTCCATTTTGATTCTGAATCCACACGAACTTGAATTGAAAAATCTCAGATNATA
ATTAAAGGAGCATATTCTTCCTCCGTGTATTTATNAGCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGT

Last Updated on 06.12.2018 16:11