Sequences from confirmation amplicon


Confirmation sequences for line 520G04 and hit At2g45560 are shown.
Line ID 520G04
Locus At2g45560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18776192
Primers used for PCR ZZ62 - 8474, show primer details
   
Sequence Name 78-K021740-0022-520-G04C-ZZ62
GenBank Accession LN513616 [GenBank]
Sequencing primer gene specific primer, ZZ62: 5'-TGCTTGCTTCTCTTCTTTATTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K021740-0022-520-G04C-ZZ62
GATTTGGACATGGACGAGACCTTTGGTCTAACTTTGCATAAGACCAACCCGTTACATGCC
GTACCCGTCAAGAAACGCGCCAATATTAATTAGTCGTGGTTTTATAATTAAAAATAAACA
GAGAAATAATCTCCAAAGCTTTGTTTATTACCAAAAACTATCTGTTTTCGGTTCTAGAGT
TTGTTTGTTTCTCTCTCTTGAGGTGGCTAGAGCTTGAAGAAGTCAAAAGTATCCATATTG
ATGCCTAGCTTCGGTCCTCGGTTTAGGCTAGGAAGGAAACGAAGAAGTTTCTCATGCGCC
ATCGTGCGCGAATCCGCCTTCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAAT
TATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 77-K021740-0022-520-G04C-8409
GenBank Accession LN513471 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K021740-0022-520-G04C-8409
TTCGGAATGAGCCTTTACAATTGAATATATCCTGAGAAGGCGGATTCGCGCACGATGGCG
CATGAGAAACTTCTTCGTTTCCTTCCTAGCCTAAACCGAGGACCGAAGCTAGGCATCAAT
ATGGATACTTTTGACTTCTTCAAGCTCTAGCCACCTCAAGAGAGAGAAACAAACAAACTC
TAGAACCGAAAACAGATAGTTTTTGGTAATAAACAAAGCTTTGGAGATTATTTCTCTGTT
TATTTTTAATTATAAAACCACGACTAATTAATATTGGCGCGTTTCTTGACGGGTACGGCA
TGTAACGGGTTGGTCTTATGCAAAGTTAGACCAAAGGTCTCGTCCATGTCCAAATCCTCC
GAAAGGACACCCTTCGGAAGCTTCCAGTCAAAGGAATAAAGAAGAAAAGCAAGC

Last Updated on 06.12.2018 16:11