Sequences from confirmation amplicon


Confirmation sequences for line 523E10 and hit At1g64235 are shown.
Line ID 523E10
Locus At1g64235 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23836291
Primers used for PCR NG44 - 8474, show primer details
   
Sequence Name 53-K102368-0022-523-E10C-8409
GenBank Accession LN508107 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K102368-0022-523-E10C-8409
CAGAGATGACGAATGTATAAGCAACAATCCGACGGTCTTCATGCATGTATATATAGTTGT
TTCTTTTAGATATACCTATAGCAAATATTTTGGATTCCTTTTGTTTTCTGTGCAAAACAT
GAGAATTTGTTTAGGTATTTCTTGGATAATGAAGGATTCCAATTGCATGGTATTGCGTAA
TGAACGCGTTTTTCCTTGTTAATCAACATGAACGTAATTAGTTGCTATGTAAATAACTAA
ATATAGCCTAAATGATTGGCTAAATCACTACATGAATTATTTTCAGTATTTAGAATATTT
CATTGGTTAATAATAATACACCATGATTTACAGATTTTACATTCAAATCATCATAAATTA
AATGAAAATTCAGATTGAATGAAAGNTTGGAT
   
Sequence Name 53-K102369-0022-523-E10C-NG44
GenBank Accession LN508108 [GenBank]
Sequencing primer gene specific primer, NG44: 5'-TCATCCAACTTTTCATTCAATCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K102369-0022-523-E10C-NG44
ATTTGATGTAAATCTGTAAATCATGGTGTATTATTATTAACCAATGAAATATTCTAAATA
CTGAAAATAATTCATGTAGTGATTTAGCCAATCATTTAGGCTATATTTAGTTATTTACAT
AGCAACTAATTACGTTCATGTTGATTAACAAGGAAAAACGCGTTCATTACGCAATACCAT
GCAATTGGAATCCTTCATTATCCAAGAAATACCTAAACAAATTCTCATGTTTTGCACAGA
AAACAAAAGGAATCCAAAATATTTGCTATAGGTATATCTAAAAGAAACAACTATATATAC
ATGCATGAAGACCGTCGGATTGTTGCTTATTACATTCGTCATTCTCTTGGCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATA

Last Updated on 06.12.2018 16:11