Sequences from confirmation amplicon


Confirmation sequences for line 524F10 and hit At5g19200 are shown.
Line ID 524F10
Locus At5g19200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6459884
Primers used for PCR MJ60 - 8474, show primer details
   
Sequence Name 34-K029914-022-524-F10C-8409
GenBank Accession LN501291 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K029914-022-524-F10C-8409
CCATTTTCATAAAATTTATCTTAAAAAAAATGTAAAAGATTCATTGTTTACCTTGTTCAA
ACCCGGGTGTATCAGTGTCAGGAGGAAACAAGAGAGTCACATGAATGCCATCAGAAATAA
CTTCTTGCTGCAATGCTTGCGCTAAACCCTGAAGCCCAAACTTGCTCGCTGAATATGCAG
TGTAACCATATATACCTGCCTGAAAACGTAAAAACACACGATACATCACAAAGTGCTCTC
GACTTCCACAAGTGCTTCCATTTTTACAAACTCAATTCAACAATTACCAAACTGCTTTAA
ATTCTTAATCTAATCTGCGAGTTATGATTCTATGATACGGCGTTAAGAGTGATAGAGATA
CGAACCTGACCAGCTTGAGAGGACACAAGAGAAATGGAAGCAGGACCACGACCTTCCCTA
GCTTTCATAGCAGGTAAAGCCGCCTTAATCACATTGAAGCTCCCAGTCAGATTCACATCA
ATCATAAACTTAACCTCCTCAGGACTCTGTTTCTCAAGCTCTTTCCCAATAAACA
   
Sequence Name 34-K029914-022-524-F10C-MJ60
GenBank Accession LN501292 [GenBank]
Sequencing primer gene specific primer, MJ60: 5'-GTGTTTATTGGGAAAGAGCTTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K029914-022-524-F10C-MJ60
TATGATTGATGTGAATCTGACTGGGAGCTTCAATGTGATTAAGGCGGCTTTACCTGCTAT
GAAAGCTAGGGAAGGTCGTGGTCCTGCTTCCATTTCTCTTGTGTCCTCTCAAGCTGGTCA
GGTTCGTATCTCTATCACTCTTAACGCCGTATCATAGAATCATAACTCGCAGATTAGATT
AAGAATTTAAAGCAGTTTGGTAATTGTTGAATTGAGTTTGTAAAAATGGAAGCACTTGTG
GAAGTCGAGAGCACTTTGTGATGTATCGTGTGTTTTTACGTTTTCAGGCAGGTATATATG
GTTACACTGCATATTCAGCGAGCAAGTTTGGGCTTCAGGGTTTAGCGCAAGCATTGCAGC
AAGAAGTTATTTCTGATGGCATTCATGTGACTCTCTTGTTTCCTCCTGACACTGATACAC
CCGGGTTTGAACAAGGTAAACAATGAATCTTTTACATTTTTTTTAAGATAAATTTTATGA
CAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTAT
AGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11