Sequences from confirmation amplicon


Confirmation sequences for line 526E04 and hit At1g27610 are shown.
Line ID 526E04
Locus At1g27610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9607135
Primers used for PCR JL78 - 8474, show primer details
   
Sequence Name 04-K101382-0022-526-E04C-OriL
GenBank Accession LN487850 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K101382-0022-526-E04C-OriL
GGGCACTTAATGTTTCTCCATGAACCCGTCATTAATGAAGGATTATAAATAAATGTGATG
AATATACTGTTCTTCGTCTCCATCCTTCTGATCAAACGACTTGGATGTACAAATCCAAGG
TACTCTGAAGCCTACACACCACATAGGTGTACAAATCCATAAAATTTGAGCAATAACCCG
ATAATATAGCAGAAAGCAAAAAAATGTAATCCAAAACCTAAGAAAGTCCTATAAATGATA
ATCATATAAGCACATACCTTGAAAAGCTCGAAGAGCTTCTTAGGATCTTCTTCTTTTTTG
TTGAATTCCTCGCGGATCCACTTCTCATCCTTAGTGGAGAAGGGTTTGTAGGAATCTTCT
TCTGGTTGATAGTTGCTTTGAAAGAAGAAGCTCCAACAAGCATGTGTTATAAGCTGTCTT
TCTGGTGTGGGAAACAGAACTCTTATATGAATATNTATAAAGGTGTTCATTAGTTTCCTT
NCATGTTTACNATCTTCCTCATGATTTCACTAGAGACATCCCGAGTACAAACG
   
Sequence Name 04-K101388-0022-526-E04C-JL78
GenBank Accession LN487851 [GenBank]
Sequencing primer gene specific primer, JL78: 5'-CTCTCGACTCTCTACGCTTTGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K101388-0022-526-E04C-JL78
AAATCATCTATCATGACTGGAGGAGAGTATGCAAAAGTAAACATAACTTGCAATGACTCA
GTTGTTAAAATCCTTGATCCTTGTTCTATGGCTGAAGTATCCACAAAAGTTTCAGAAATT
CTTAAGAAATCATCCGATCCCGAAGTCGTTTGTACTCTGGATGTCTCTAGTGAAATCATG
AGGAAGATTGTAAACATTGAAGGAAACTAATGAACACCTTTATAAATATTCATATAAGAG
TTCTGTTTCCCACACCAGAAAGACAGCTTATAACACATGCTTGTTGGAGCTTCTTCTTTC
AAAGCAACTATCAACCAGAAGAAGATTCCTACAAACCCTTCTCCACTAAGGATGAGAAGT
GGATCCGCGAGGAATTCAACAAAAAAGAAGAAGATCCTAAGAAGCTCTTCGAGCTTTTCA
AGGTATGTGCTTATATGATTATCATTTATAGGACTTTCTTAGGTNTTGGATTACATTTTT
TTGCTTTCTGCTATATNATCGGGTTATTGCTCANATTTTATGGATTTGTACACCTATNTG
GTGTGTAGGCTTCAGAGTACCTGGAATTTGTACATCCNAGTCGTTTGATCAGAAGGATGG
AGACGAAGAACAGTATATTCATCACATTTA
Primers used for PCR JL77 - 8474, show primer details
   
Sequence Name 03-K101382-0022-526-E04C-8409
GenBank Accession LN487417 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K101382-0022-526-E04C-8409
TTACATTGAAATATACTCATTGCTCATTGCAAGTCTTTACAATTTTCATAGCCATCTTTT
TTCCTTTGTAGCTCACTTTAGAAGAACTTTAGAATCGTGTATCGTTTATCTGATACCGTT
TGCTTCACAACATACGTTGAGTCGATAAATTGATTGTTTTTATTTTATGTTTTTATCTGT
TTTTTGCCATGAGATCAAATCAATAGATTTAACATCTTGTAAACCTAAACATTTTTATTT
TAATAAATAATTAAAAATCACTACATAAATTTTTAATATTTTGACACAAAAACTTATGTA
AATCTATTTTTGAAACTAGATATCTGCTTGTACCGTTAAAAATAGATNTAACAAAACAAC
TGAGAAATTTTTTTGACTAAGCTTTCAAACATACAATACTAAATCTAATTCTACTTGAAA
AAAAAAGAAAAAGAAAAAAAACACCACAGCNAAAAAAATTAATCGCTTAATCAAATATTA
CGTC
   
Sequence Name 03-K101388-0022-526-E04C-JL77
GenBank Accession LN487418 [GenBank]
Sequencing primer gene specific primer, JL77: 5'-GCATTTCTTACATCATTGCAACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K101388-0022-526-E04C-JL77
TCCGTTTTGGTTTTTACGGTGCAAAATATTAGACAAAATTTTAAAACATGTGTAATTTAC
AGTCCTTTTTCGAAAGTTTGGGTTATTTTAATACCAGTTTTCTATAATATCAAGCTTGGC
GACGTAATATTTGATTAAGCGATTAATTTTTTTTGCTGTGGTGTTTTTTTTCTTTTTCTT
TTTTTTTCAAGTAGAATTAGATTTAGTATTGTATGTTTGAAAGCTTAGTCAAAAAAATTT
CTCAGTTGTTTTGTTAAATCTATTTTTAACGGTACAAGCAGATATCTAGTTTCAAAAATA
GATTTACATAAGTTTTTGTGTCAAAATATTAAAAATTTATGTAGTGATTTTTAATTATTT
ATTAAAATAAAAATGTTTAGGTTTACAAGATGTTAAATCTATGGATTTGATCTCATGGCA
AAAAACAGATTANAACATAAAATAAAAACAATCAATTTATCGACTCAACGTATGTTGTGA
AGCANACGGTATCAGATAAACGATACACGATTCTNAAGTTCTTCTAAAGTGAGCTACAAA
GGGAAAAAGATGGCTATGAAAAT

Last Updated on 06.12.2018 16:11