Sequences from confirmation amplicon


Confirmation sequences for line 528C10 and hit At3g51780 are shown.
Line ID 528C10
Locus At3g51780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19207136
Primers used for PCR LS01 - 8474, show primer details
   
Sequence Name 23-K024451-022-528-C10C-8409
GenBank Accession LN496401 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K024451-022-528-C10C-8409
CATGTAGATTTCCCGGACATGAAGCCATCATACTCAGCTGCAGCTTTGCTCTGATCCACA
AACGGATTTGAACATCTTGCCTTCAACTTATCCACAGCCTCCTGCAAGTTTTGGATTCTA
CGTACCTGCCAACACAAACCACAACTCATATATCTTCCAACTCTATTAACACTCTTACCG
TGTAAACATGCTTAACAAAGCAAGAAAACTAACCTCAGCCTTACGCTGTACTTTAGCGTC
CCCTTCAGCCTCAATGCCATCCAATTTGAGCAGCTGCCTCATAAGAAGCTCTGCAGCCAT
GTCAAACTCCCGCACCGCAACTTGCGTCCCTCCATTCACAGCAACTTCTAAAGCAACAAC
CTAATTACCAAAAAAAACTAGCTTAAGCACAATCCTTCCGTCATCTCCAAAAGAAATACA
GGCTTATAAAAACTCACTCTATCCGAGAGCTTATCGACCTCTCCTGTAACCGCGTTAACA
GCAGCAATAGCTTTTTCCATCTCTTTAGTTACCACAGGAGGCTGTTGTTCCACTCTCTTA
TTCGTATCCTCAACAACAACAACAAGCTTAGACGCATCCTTAACACCAGCAGCTTGCAAT
TGTTCAGCATCATCTCTCTCAACTCCTCTGAACAAGATCTTCAATTCACTAGCT
   
Sequence Name 24-K024451-022-528-C10C-LS01
GenBank Accession LN496841 [GenBank]
Sequencing primer gene specific primer, LS01: 5'-AAAACTGGATTGGAAGCTAGTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K024451-022-528-C10C-LS01
AAGATCTTGTTCAGAGGAGTTGAGAGAGATGATGCTGAACAATTGCAAGCTGCTGGTGTT
AAGGATGCGTCTAAGCTTGTTGTTGTTGTTGAGGATACGAATAAGAGAGTGGAACAACAG
CCTCCTGTGGTAACTAAAGAGATGGAAAAAGCTATTGCTGCTGTTAACGCGGTTACAGGA
GAGGTCGATGAGCTCTCGGATAGAGTGAGTTTTTATAAGCCTGTATTTCTTTTGGAGATG
ACGGAAGGATTGTGCTTAAGCTAGTTTTTTTTGGTGATTAGGTTGTTGCTTTAAAACTTG
CTGTGAATGGAGGGACGCAAGTTGCGGTGCGGGAGTTTGACATGGCTGCAGAGCTTCTTA
TGAGGCAGCTGCTCACATTGGATGGCATTGAGGCTGAAGGGGACGCTAAAGTACAGCGGA
AGGCTGAGGTTAGTTTTCTTGCTTTGTTAAGCATGTTGACACGGTAAGAGTGTTACTACA
GTTGGAGGATATAAATACTTGTGGTTTGTGT

Last Updated on 06.12.2018 16:11