Sequences from confirmation amplicon


Confirmation sequences for line 529C11 and hit At1g33120 are shown.
Line ID 529C11
Locus At1g33120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12011499
Primers used for PCR KR21 - 8474, show primer details
   
Sequence Name 37-K030602-022-529-C11C-8409
GenBank Accession LN502501 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K030602-022-529-C11C-8409
CGGTAACGATATCGAGCTTGTTTCAGGTCGTGCGCTCTGATTCAAACCAGGTACTCCATT
TCTTTAAACTAGTAGATATCACAATTTTGTTTTAATGATCTGAAGATATCACAATTCTCT
CATAGTCTACTATATCCACAACAACTTGAAAATAATCTTGGGTCGAAAGATAGAGCCGTT
GTGTAGTATAGGAGACGTAGAATTCTTTTGTTTTGGTTGAAGTTTGAGTTTGGGATCACA
TTTTGCATAGTG
   
Sequence Name 76-K030602-022-529-C11C-KR21
GenBank Accession LN513321 [GenBank]
Sequencing primer gene specific primer, KR21: 5'-TGAATCAGTTCGATTTCACTATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K030602-022-529-C11C-KR21
CAACGGCTCTATCTTTTCGACCCAAGAATAATTTCAAGTCGTCGCGGATATAGTAGACTA
CGAGAGAATCGTGATATCTTCAGATCATTAAAACAAAATCGTGATATCTACTAGTTAAAG
AATGGAGTACCTGGTTGATCAGAGCGCACGACCTTGAAACAAGCTCGATATCGTTACCGT
CAAGAACAATCTCATCCTTCACCTTCTCAGATCGAACAATGGTTACACCATCCAACATCT
CTACCTTCCTCACCTATTATTCCAATAAACACATTTCTATTTTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT

Last Updated on 06.12.2018 16:11