Sequences from confirmation amplicon


Confirmation sequences for line 532E11 and hit At4g23010 are shown.
Line ID 532E11
Locus At4g23010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12060575
Primers used for PCR MP34 - 8474, show primer details
   
Sequence Name 07-K023022-022-532-E11C-8409
GenBank Accession LN489079 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K023022-022-532-E11C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATTAAGATTAAAATT
AGATATGACATGAAGCCATTTACACTGATAGCTATCATTATCTAAAATCACATTTCGTTG
TGTGTAGATAACAACGGCTAGGAAAGGGGTTACATTGTTGCTGTCGTATTTGATATTCAC
AAAGCCGTTAACGGAACAGCACGGATCAGGCTTGCTGTTGATAGCAATGGGGATTGTATT
GAAGATGGTTCCAATGGATAGTAAAGCTCCGGCCAAGATTCCGGCGAGACCAGCGGTTAG
GATCGCAGGAGGCGATGGTGATAGAGAAGAGGACGAAGAAAGGAAGTCATTGGTTTGAGA
ATGTGCCATGTATTTATATATATTTTATCTCACAAATTCAAAGGGTTTATAGAATAATGT
TGGATGAAGGAG
   
Sequence Name 08-K023022-022-532-E11C-MP34
GenBank Accession LN489545 [GenBank]
Sequencing primer gene specific primer, MP34: 5'-TCTATTTTGTGTCTCCTTCATCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K023022-022-532-E11C-MP34
TATTCTATAAACCCTTTGAATTTGTGAGATAAAATATATATAAATACATGGCACATTCTC
AAACCAATGACTTCCTTTCTTCGTCCTCTTCTCTATCACCATCGCCTCCTGCGATCCTAA
CCGCTGGTCTCGCCGGAATCTTGGCCGGAGCTTTACTATCCATTGGAACCATCTTCAATA
CAATCCCCATTGCTATCAACAGCAAGCCTGATCCGTGCTGTTCCGTTAACGGCTTTGTGA
ATATCAAATACGACAGCAACAATGTAACCCCTTTCCTAGCCGTTGTTATCTACACACAAC
GAAATGTGATTTTAGATAATGATAGCTATCAGTGTAAATGGCTTCATGTCATATCTAATT
TTAATCTTAATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC
GGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11