Sequences from confirmation amplicon


Confirmation sequences for line 536D04 and hit At2g29410 are shown.
Line ID 536D04
Locus At2g29410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12616926
Primers used for PCR CT17 - 8474, show primer details
   
Sequence Name 73-K105358-0022-536-D04C-CT17
GenBank Accession LN512898 [GenBank]
Sequencing primer gene specific primer, CT17: 5'-TTTGAAGACGATTTCTCACCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K105358-0022-536-D04C-CT17
CACACCTCCTTGTGATGATGTTGATGTTGATGTTTATGATTATCATGATGGTGATGATGG
TCGTGGTGGTGGTGGCTATGGTTATGTCCTAACCAAAGGACCATAACCAAGTTCATGAAA
AACCCGAAAGCCGAAATCCCAAACATGATTTCTCCATTAACCTCTCTGCTTCTGCTAAGT
AGTCTTTGAATGGCTTCATGGATTATCACTCCAGAGACAAGCCATATGAGCTGAACAGAC
AAGAACGCCGCTAAAACTTCAAGCCGTTTAAACCCGAACGAGTTTCGCGGATTAGCTTCC
CAGCTCGAGACCTTGATCGCCAACAAAGAGACACACAAACCCGCCACATCCGACAACAAA
TGCGCAGCATCTGTCATAACCGCAAGACTATTCGCCTTAAAACCTCCCACAATCTGTACG
GACATAACGATGAGGTAAAGAAAAATAAGGCTGCTTAGTCTTCTAGTCGATTCTTCGCGT
TCTTTCGTCTCGGAAACGCAATGTTCTTGTCTCCATATTGATCCATCATACTCATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGGTTATT
   
Sequence Name 57-K105359-0022-536-D04C-8409
GenBank Accession LN509416 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K105359-0022-536-D04C-8409
TTACATTGAATATGAGTATGATGGATCAATATGGAGACAAGAACCTTGCGATTTCCGAGA
CGAAAGAACGCGAAGAATCGACTAGAAGACTAAGCAGCCTTATTTTTCTTTACCTCATCG
TTATGTCCGTACAGATTGTGGGAGGTTTTAAGGCGAATAGTCTTGCGGTTATGACAGATG
CTGCGCATTTGTTGTCGGATGTGGCGGGTTTGTGTGTCTCTTTGTTGGCGATCAAGGTCT
CGAGCTGGGAAGCTAATCCGCGAAACTCGTTCGGGTTTAAACGGCTTGAAGTTTTAGCGG
CGTTCTTGTCTGTTCAGCTCATATGGCTTGTCTCTGGAGTGATAATCCATGAAGCCATTC
AAAGACTACTTAGCAGAAGCAGAGAGGTTAATGGAGAAATCATGTTTGGGATTTCGGCTT
TCGGGTTTTTCATGAACTTGGTTATGGTCCTTTGGTTAGGACATAACCATAGCCACCACC
ACCACGACCATCATCACCATCATCATAATCATAAACATCAACATCAACATCATCACAAGG
AGGTGGTGGCAGAAGAGGAGGAGGAGGAAATGAATCCTTTAAAAGGTGAGAAATCGTCTT
CAA

Last Updated on 06.12.2018 16:11