Sequences from confirmation amplicon


Confirmation sequences for line 537B05 and hit At3g47810 are shown.
Line ID 537B05
Locus At3g47810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17639602
Primers used for PCR HP75 - 8474, show primer details
   
Sequence Name 71-K033553-022-537-B05C-8409
GenBank Accession LN512497 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K033553-022-537-B05C-8409
GAAACAAAGGATCTTCCATGGGCGATCGCTGAGAAGATCCGCAAGAACAAAAACATCAAA
TTCCTTGAAATCAGGAATCTTATTCGATAGTCTATTTGAGCCCTTGATCTGGTAAGCTCT
GTCTGATCTTCTAAAGTTTCGCTTTTTAACTTTTGGGGAAATCATCCACCTTTGTATCAA
GTTTCTGGCAATTTCTTCTCTGTAGTTAGAATCATTCAATAAGCCCTTTAATCTCTTCGA
CGCAGCTAGTGTTGAATTACATCTGGGTTTTGTCTACTTTGTGTCAAAACCATGCTTTTA
TGTTTGATTTTCGCGAAGTATGATTTAGGTTCGATTTTAGTGAAAGAAAGACTTAACAGT
TGTAGTTAACTGAAACTATGCGATATCTGATTACGTTGGTGTTATGATTTTATTGGAAGG
GTTTTGGAAATGATTCCACATTGTATTGTAGATCTCATTGACTTTTGGTTTTTGATCTGT
TTCTCAATCCAGATTCTTTATACTTTAGTTTAACAAGTGACAAACTTTGATCTTTGATGG
GGTTCAC
   
Sequence Name 71-K033553-022-537-B05C-HP75
GenBank Accession LN512498 [GenBank]
Sequencing primer gene specific primer, HP75: 5'-GGTGAACCAATCAAAGATCAAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K033553-022-537-B05C-HP75
GATCTGGATTGAGAAACAGATCAAAAACCAAAAGTCAATGAGATCTACAATACAATGTGG
AATCATTTCCAAAACCCTTCCAATAAAATCATAACACCAACGTAATCAGATATCGCATAG
TTTCAGTTAACTACAACTGTTAAGTCTTTCTTTCACTAAAATCGAACCTAAATCATACTT
CGCGAAAATCAAACATAAAAGCATGGTTTTGACACAAAGTAGACAAAACCCAGATGTAAT
TCAACACTAGCTGCGTCGAAGAGATTAAAGGGCTTATTGAATGATTCTAACTACAGAGAA
GAAATTGCCAGAAACTTGATACAAAGGTGGATGATTTCCCCAAAAGTTAAAAAGCGAAAC
TTTAGAAGATCAGACAGAGCTTACCAGATCAAGGGCTCAAATAGACTATCGAATAAGATT
CCTGATTTCAAGGAATTTGATGTTTTTGTTCTTGCGGATCTTCTCAGCGATCGCCCATGG
AAGATCCTTTGTTTTCCCTTTTCTTCTCTCTCGATGATCTTAGGAAAGGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11