Sequences from confirmation amplicon


Confirmation sequences for line 541D06 and hit At3g12670 are shown.
Line ID 541D06
Locus At3g12670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4024608
Primers used for PCR KG19 - 8474, show primer details
   
Sequence Name 92-K038034-022-541-D06C-KG19
GenBank Accession LN515170 [GenBank]
Sequencing primer gene specific primer, KG19: 5'-TCACCAATTTTAATGGAAGTGACA-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K038034-022-541-D06C-KG19
GTCCAATGCTACTAGCAGTGACACCTTTGCCAAGACCACTCACCACTCCTCCTGTCACCA
AAACGTACTTCATAATCGTTTTTGTCTCTGCTTCTTCTTCTTCACCTAAATCATCAAAAA
ACACAGAACACGGTAAGGAAAGATAAGACTTTGAGAAGATATCTCTACCAAACATAGAAG
AGATTATCGGGTATGAGTCGAAACAATAAAGTGAGCAGAAGAAATTTACGAATTCTACCT
TTCACCAACAAAAATGGCCTTCTGGATTTTCAAGGCGGCGGCTTATAAGCTAACCTGCGA
GACCCTCACTCTTATTTAAACCCAAATTGAAAGCAAATCAAAATAGTTTTTTTATTTGGT
CAAAAGTTTTTTAATCTCAAAATAATACATCAACATTGGGCTTGAGTAAGAAAATAAAAT
AAAAAGGGACTGAAACGGTAAATTTCAAAACCATGGTAAATTTATTTTTCTGCTCCAGAC
TCTTGAGATTAGATTTAGGGTTTGTGACACTCGTGTTTGTGTTGTGGAAGTTTGCATAAT
TTTGGTAAGATAGGCCCAAAAGAGTATTTTGGATTATAGAAGTATTTTGGACCTAACAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GACGTGTCTAC
   
Sequence Name 36-K038034-022-541-D06C-8409
GenBank Accession LN502138 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K038034-022-541-D06C-8409
CCATTTACAATTGAATATATCCTGTTAGGTCCAAAATACTTCTATAATCCAAAATACTCT
TTTGGGCCTATCTTACCAAAATTATGCAAACTTCCACAACACAAACACGAGTGTCACAAA
CCCTAAATCTAATCTCAAGAGTCTGGAGCAGAAAAATAAATTTACCATGGTTTTGAAATT
TACCGTTTCAGTCCCTTTTTATTTTATTTTCTTACTCAAGCCCAATGTTGATGTATTATT
TTGAGATTAAAAAACTTTTGACCAAATAAAAAAACTATTTTGATTTGCTTTCAATTTGGG
TTTAAATAAGAGTGAGGGTCTCGCAGGTTAGCTTATAAGCCGCCGCCTTGAAAATCCAGA
AGGCCATTTTTGTTGGTGAAAGGTAGAATTCGTAAATTTCTTCTGCTCACTTTATTGTTT
CGACTCATACCCGATAATCTCTTCTATGTTTGGTAGAGATATCTTCTCAAAGTCTTATCT
TTCCTTACCGTGTTCTGTGTTTTTTGATGATTTAGGTGAAGAAGAAGAAGCAGAGACAAA
AACGATTATGAAGTACGTTTTGGTGACAGGAGGAGTGGTGAGTGGTCTTGGCAAAGGTGT
CACTGCTAGTAGCATTGGACTTCTCCTTCAAGCATGTGGTCTTCGTGTCACTTCCATT

Last Updated on 06.12.2018 16:11