Sequences from confirmation amplicon


Confirmation sequences for line 544E07 and hit At2g22630 are shown.
Line ID 544E07
Locus At2g22630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9619178
Primers used for PCR SZ06 - 8474, show primer details
   
Sequence Name 47-K021853-022-544-E07C-8409
GenBank Accession LN506059 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K021853-022-544-E07C-8409
ATGCACATTGATTGTAGTGATATCTTATACACTCCCATTTATCACTCTTTCAGTTTTCCA
ACGTTATACTTGTTTTTAGCTTTAATTACTATCTTCTCTACTCAAATGTTTCTAAATTTG
GCAAAGACACACTGTCAGCCACATACACAATTTACAACCAATGACACACATGTCCACACA
CTGCCATGAGCCTACTTCAAATTAGTGTGTGTTTTCATCTTTACACTAGTCATCGTCTTT
AATTAGTTTTCTTTTGCTTTGGCATCCTAAGCTCCTTTCAAACTAAGTTTCCTTTGAAAA
TTAAGTTTCCTTAGCTCATACATTTATCACCTTGTATATATACACAGAAATATAGAGAGC
GTTATCGCCACAAGATGCAGAAAAATGTATACTACTACAAATGACTAAATGCACGCTACC
AAACACAAATCATTGAATTAATCCAAACCCTCTTAGAGAAATAGCCTATATTCTTGTAGT
GAACCCACACATGATATTATATAAGTACGTACATACCTATATATAGTTCCATTTATTATA
GATATACAAATACATAGATAGATGTAGATCTATAGGTATGTAGATGTTAAAAGATCTAAG
AATTGAATAAAGGAAGAGAGAAACCTGGAGCTGGCAAAGTCATAGAGCTTGTCAGTGTTG
   
Sequence Name 48-K021853-022-544-E07C-SZ06
GenBank Accession LN506381 [GenBank]
Sequencing primer gene specific primer, SZ06: 5'-CTCATCATTTTCTCCAACACTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K021853-022-544-E07C-SZ06
GTTTCTCTCTTCCTTTATTCAATTCTTAGATCTTTTAACATCTACATACCTATAGATCTA
CATCTATCTATGTATTTGTATATCTATAATAAATGGAACTATATATAGGTATGTACGTAC
TTATATAATATCATGTGTGGGTTCACTACAAGAATATAGGCTATTTCTCTAAGAGGGTTT
GGATTAATTCAATGATTTGTGTTTGGTAGCGTGCATTTAGTCATTTGTAGTAGTATACAT
TTTTCTGCATCTTGTGGCGATAACGCTCTCTATATTTCTGTGTATATATACAAGGTGATA
AATGTATGAGCTAAGGAAACTTAATTTTCAAAGGAAACTTAGTTTGAAAGGAGCTTAGGA
TGCCAAAGCAAAAGAAAACTAATTAAAGACGATGACTAGTGTAAAGATGAAAACACACAC
TAATTTGAAGTAGGCTCATGGCAGTGTGTGGACATGTGTGTCATTGGTTGTAAATTGTGT
ATGTGGCTGACAGTGTGTCTTTGCCAAATTTAGAAACATTTGAGTAGAGAAGATAGTAAT
TAAAGCTAAAAACAAGTATAACGTTGGAAAACTGAAAGAGTGATAAATGGGAGTGTATAA
GATATCACTACAATCAATTGTGCATTTTTAAGGAAGATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGT
GTCTAC

Last Updated on 06.12.2018 16:11