Sequences from confirmation amplicon


Confirmation sequences for line 547H03 and hit At5g03740 are shown.
Line ID 547H03
Locus At5g03740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:983459
Primers used for PCR IF09 - 8474, show primer details
   
Sequence Name 27-K032519-022-547-H03C-8409
GenBank Accession LN498270 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K032519-022-547-H03C-8409
TTACAATTGAATATATCCTGAAGCACATAAAAGAGACTGTCATGTTACCTTCTTTGGGGT
TTCCTCTTCTGAGGAGTCATCTTCTTCGTCGTCTGATGAGTCATCTTCCTCACTGTCAGA
TTCAGCAGTAACCTTCTCTTCCTGAAACAAAGCAGCCATGATAAGGATTAAAGAAATGTG
AGAATGGGCTAAAACACAATAGGTAATCAAGGGTTCATGTGAAAATGCAAATATAGAAGT
TACAAACATACTTAAGATAACTCTAGTACCACTGTCTATAGGTTTAATCAACATGTCACT
GAGCCTAAGTAAAATTCTATAACAAATTGAGCTAGTATATATAGATAAAAGTACCTCCTC
GTCTCCAGAGTTTTCACTATCATCATCATCTGAAGAATCTTCTTCACTACCGTCAGCGTC
ATCATCTTGCTTGGCTTTGACATCTTCATTTGGCAACTGAAAGTTCACCTGTTTCGCAGC
AGATTTCGGAGCAGCTGGCTCAACCAAGGCCAAATTAAATTT
   
Sequence Name 27-K032519-022-547-H03C-IF09
GenBank Accession LN498271 [GenBank]
Sequencing primer gene specific primer, IF09: 5'-TAAATTTATTTGGCCTTGGTTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K032519-022-547-H03C-IF09
GTGACTTTCGTTGCCAAATGAAGATGTCAAAGCCAAGCAAGATGATGACGCTGACGGTAG
TGAAGAAGATTCTTCAGATGATGATGATAGTGAAAACTCTGGAGACGAGGAGGTACTTTT
ATCTATATATACTAGCTCAATTTGTTATAGAATTTTACTTAGGCTCAGTGACATGTTGAT
TAAACCTATAGACAGTGGTACTAGAGTTATCTTAAGTATGTTTGTAACTTCTATATTTGC
ATTTTCACATGAACCCTTGATTACCTATTGTGTTTTAGCCCATTCTCACATTTCTTTAAT
CCTTATCATGGCTGCTTTGTTTCAGGAAGAGAAGGTTACTGCTGAATCTGACAGTGAGGA
AGATGACTCATCAGACGACGAAGAAGATGACTCCTCAGAAGAGGAAACCCCAAAGAAGGT
AACATGACAGTCTCTTTTATGTGCTTCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
Primers used for PCR FQ08 - 3144, show primer details
   
Sequence Name 39-K033039-022-547-H03C-3144
GenBank Accession LN503260 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K033039-022-547-H03C-3144
TCCACTATCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGAACCTATATTTGTTCTATCTTATCTTCCTTTGGGAGCAATTTCTCTGC
TTCTGTGGTCAAGCTCGAATCTGATTCTAAGTTTTCTTGGTTTATAAAAGCCTGAAGAAC
CCAAGAAGAGGTCTGCAGAACCCAACTCCTCCAAGAACCCTGCGTCCAACAAGAAGGCTA
AGTTTGTAACTCCTCAGAAAACAGGTGATTAAATCCTTTTTACCAATCTCCCCATTGAAT
ACTTATTTTAACGATAGAAAGGAAGATTGATTTGTTTAATTAAAAAACATTCGGACAAAG
CAGATTCGAAGAAACCGCATGTCCATGTTGCAACTCCTCATCCCTCAAAACAGGCGGGTA
AGAATTCTGGTGGAGGCAGCACTGGAGAGACATCGAAGCAGCAGCAGACACCGAAGTCTG
CAGGAGCGTTTGGGTGCAAGTCGTGCACCAGAACCTTTACTTCGGAAATGGGATTGCAGT
CTCACACAAAGGCCAAACACAGTGCAGCTGCTTGAGAAGAAGAAGCAGCATGATGACTAC
TCTCTATAATATCTACTCTAGAACTTTGATGTCTTTTGGTACTTAAATCTGGAACTTGCG
AGTGAGCCAG
   
Sequence Name 07-K033039-022-547-H03C-FQ08
GenBank Accession LN489164 [GenBank]
Sequencing primer gene specific primer, FQ08: 5'-AAAAGACCAACTAAAACTGGCTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K033039-022-547-H03C-FQ08
AAGACATCAAAGTTCTAGAGTAGATATTATAGAGAGTAGTCATCATGCTGCTTCTTCTTC
TCAAGCAGCTGCACTGTGTTTGGCCTTTGTGTGAGACTGCAATCCCATTTCCGAAGTAAA
GGTTCTGGTGCACGACTTGCACCCAAACGCTCCTGCAGACTTCGGTGTCTGCTGCTGCTT
CGATGTCTCTCCAGTGCTGCCTCCACCAGAATTCTTACCCGCCTGTTTTGAGGGATGAGG
AGTTGCAACATGGACATGCGGTTTCTTCGAATCTGCTTTGTCCGAATGTTTTTTAATTAA
ACAAATCAATCTTCCTTTCTATCGTTAAAATAAGTATTCAATGGGGAGATTGGTAAAAAG
GATTTAATCACCTGTTTTCTGAGGAGTTACAAACTTAGCCTTCTTGTTGGACGCAGGGTT
CTTGGAGGAGTTGGGTTCTGCAGACCTCTTCTTGGGTTCTTCAGGCTTTTATAAACCAAG
AAAACTTAGAATCAGATTCGAGCTTGACCACAGAAGCAGAGAAATTGCTCCCAAAGGAAG
ATAAGATAGAACAAATATAGGTTCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTT
CCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCA
GTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCC
ACGATGCTCCTCGT

Last Updated on 06.12.2018 16:11