Sequences from confirmation amplicon


Confirmation sequences for line 550G09 and hit At1g79560 are shown.
Line ID 550G09
Locus At1g79560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29929614
Primers used for PCR HP71 - 8474, show primer details
   
Sequence Name 18-K033718-022-550-G09C-HP71
GenBank Accession LN494207 [GenBank]
Sequencing primer gene specific primer, HP71: 5'-TCATCAGGTCTATTGGTAGCACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K033718-022-550-G09C-HP71
TATCGATACCTGTCTTCTCTTTCCTGCAGGTGGTACAATATTAAGTATGAAAGCCTTGAT
ACAAACCCGATGAAAACATGTACATATCACTAAGATTTATAAACAAGTGCAGATTCTCTT
ACTCCCCATCGAGTTGCGCAATTAAAGCTTCAAAAGTTGCTCTTCTCCGTGGATCTTTTC
TAGCATGTCTACCAGCAATAGCATCTATTTCATCCACAAACACAAAAGCAGGGGCCTGGA
GATAGTATATAAAATTAGATCCACAATAATAGTGTGAGTAGAAGACAACTATACCGTATG
AGCTTTAAAACACATAACTAAGAACCTTACATTTCGTCTCGCGATTGAAAACATCTCATT
GATCTTTGCAGCACCACTTTTTTCACTGTCTGTGAACTCCGCACCAGATGCAAACACAAA
TGGTAGCCCACTTTCCTTTGCAAGTGTTCGCGCAAAAAGTGTTTTTCCAGTTCCAGGAGG
TCCAGAAAGAAGCACACCCTGACTCAAAAATGAAAACAGAGGAGAGTTTGAGCTTAGCTT
AGACAGTTAAATGGAATAAACCAATATCCACAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 56-K033718-022-550-G09C-oriL
GenBank Accession LN509022 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K033718-022-550-G09C-oriL
TTACATTGAATATATCCTGTGGATATTGGTTTATTCCATTTAACTGTCTAAGCTAAGCTC
AAACTCTCCTCTGTTTTCATTTTTGAGTCAGGGTGTGCTTCTTTCTGGACCTCCTGGAAC
TGGAAAAACACTTTTTGCGCGAACACTTGCAAAGGAAAGTGGGCTACCATTTGTGTTTGC
ATCTGGTGCGGAGTTCACAGACAGTGAAAAAAGTGGTGCTGCAAAGATCAATGAGATGTT
TTCAATCGCGAGACGAAATGTAAGGTTCTTAGTTATGTGTTTTAAAGCTCATACGGTATA
GTTGTCTTCTACTCACACTATTATTGTGGATCTAATTTTATATACTATCTCCAGGCCCCT
GCTTTTGTGTTTGTGGATGAAATAGATGCTATTGCTGGTAGACATGCTAGAAAAGATCCA
CGGAGAAGAGCAACTTTTGAAGCTTTAATTGCGCAACTCGATGGGGAGTAAGAGAATCTG
CACTTGTTTATAAATCTTAGTGATATGTACATGTTTTCATCGGGTTTGTATCAAGGCTTT
CATACTTAATATTGTACCACCTGCAGGAAAGAGAAGACAGGTATCGATAGGTTTTCTTTG
AGACAAGCTGTGATATTTATCTGTGCTACCAATAGACCCTGATG
Primers used for PCR HP72 - 8474, show primer details
   
Sequence Name 19-K033718-022-550-G09C-HP72
GenBank Accession LN494664 [GenBank]
Sequencing primer gene specific primer, HP72: 5'-CCGGGGATACTAATTTTATGGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K033718-022-550-G09C-HP72
CTCTTAACATCCAGCGCTTTCTCTACAAGAAGTATAATCAGCTGTTTGATATGGCTTATG
CAGAAAATTTTATATTGGTAATCATCGTTACTGTATACCATTAACCTCGTTACTTCTTTG
TTTAGTTGCCTTCTTCCCACATATTCACACTTCTATTGATGAGTTTTAATTATCATACAG
CCGGTTGGAGATGTCAGTGAGACAAAATCAATGTATAAGGAAGTGGTACTAGGCGGTGAT
GTCTGGGATCTTCTTGATGAGCTAATGATCTACATGGGGAACCCAATGCAATACTATGAA
AAAGATGTGGCTTTTGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGG
   
Sequence Name 57-K033718-022-550-G09C-8409
GenBank Accession LN509335 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K033718-022-550-G09C-8409
TTACAATTGAATATATCCTGTCAAAGCCACATCTTTTTCATACTATTGCATTGGGTTCCC
CATGTAGATCATTAGCTCATCAAGAAGATCCCAGACATCACCGCCTAGTACCACTTCCTT
ATACATTGATTTTGTCTCACTGACATCTCCAACCGGCTGTATGATAATTAAAACTCATCA
ATAGAAGTGTGAATATGTGGGAAGAAGGCAACTAAACAAAGAAGTAACGAGGTTAATGGT
ATACAGTAACGATGATTACCAATATAAAATTTTCTGCATAAGCCATATCAAACAGCTGAT
TATACTTCTTGTAGAGAAAGCGCTTGGATGTGATAAGGAGAAGCATGGCAGATTCTCTTA
TAAACCATAAAATTAGTATCCCCGG

Last Updated on 06.12.2018 16:11