Sequences from confirmation amplicon


Confirmation sequences for line 553B01 and hit At1g12890 are shown.
Line ID 553B01
Locus At1g12890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4392157
Primers used for PCR JL48 - 8474, show primer details
   
Sequence Name 23-K101383-0022-553-B01C-8409
GenBank Accession LN496488 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K101383-0022-553-B01C-8409
GCCTTTACATTGAATATGTCACTGATAGGTAGAATGTGAGAAAGTGTTAGTGAGATTGGT
AGAAGACTCTTGTTTTTGAGAAAAAGAAGAAGAGATAGGATCAGCAACAAAATGGTTGTT
TTCAAAAGGCTGATCTTGAAGAAGACAGAGACTAGAAGATCCCCAAGTCATATGAGGCTC
CAAAGACTGTTGTGGATGTCTTTCTAAAGAGGTGTTTTCAGTGTAGAAGAAGTTTGTTGT
AGCTAGAGAGCCGCTGATGGATCGAGCAGCAACGTCATATGCAAAAGCAGCTTCTTCGGC
TGTATCAAACGTTCCAAGCCAATGTCTTTCTTTAG
   
Sequence Name 23-K101389-0022-553-B01C-JL48
GenBank Accession LN496489 [GenBank]
Sequencing primer gene specific primer, JL48: 5'-ACTAAAGAAAGACATTGGCTTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K101389-0022-553-B01C-JL48
CTGCTTTTGCTTGACGTTGCTGCTCGATCCATCAGCGGCTCTCTAGCTCCATAAACTTCT
TCTACACTGAAAACACCTCTTTAGAAAGACATCCACAACAGTCTTTGGAGCCTCATATGA
CTTGGGGATCTTCTAGTCTCTGTCTTCTTCAAGATCAGCCTTTTGAAAACAACCATTTTG
TTGCTGATCCTATCTCTTCTTCTTTTTCTCAAAAACAAGAGTCTTCTACCAATCTCACTA
ACACTTTCTCACATTCTACCTATCAGTGACATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11