Sequences from confirmation amplicon


Confirmation sequences for line 556C10 and hit At5g61380 are shown.
Line ID 556C10
Locus At5g61380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24674974
Primers used for PCR JC34 - 8474, show primer details
   
Sequence Name 04-K101743-0022-556-C10C-8409
GenBank Accession LN487859 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K101743-0022-556-C10C-8409
ATTTACATTGAATAATCCTGGTACTTAATGGTGGGACTTAATCTCCACCGTCCAATTCTA
TCCTCTTTCAACGGTCTCTGATTTACCATCTCTCTCTCTCTTTTTCGCGTCTCATAATTT
GACCATTGATTGACAAAACAAAACAATGAGTCTTCTTTCTCTTCTCACTCTTCTTGTCGG
AAATTCACCTTCTCTCTCTCTCCTCCTCATCTCACCGCCGGCGTTACGCCGGATCTCCGG
TGACTTTTGTTGAGCTTTTTTGTTGATTCATGATTCGATATAGAGAAAAAAGAATTTCCT
ATTTCAGCTCTTTGTTCTTAGATTTATCTTTTTGTTTGTTCTTATGGATAAAGGATTAGG
TTGGGGGATTAAAAGAGTAGTTTTTGTTTGGTTGTGAGATTACTTGACTTTGTTAAGGGG
ATAAATTAGGCGAC
   
Sequence Name 04-K101749-0022-556-C10C-JC34
GenBank Accession LN487860 [GenBank]
Sequencing primer gene specific primer, JC34: 5'-AAAGTCGCCTAATTTATCCCCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K101749-0022-556-C10C-JC34
AAACTACTCTTTTATCCCCCAACCTAATCCTTTATCCATAAGAACAAACAAAAAGATAAA
TCTAAGAACAAAGAGCTGAAATAGGAAATTCTTTTTTCTCTATATCGAATCATGAATCAA
CAAAAAAGCTCAACAAAAGTCACCGGAGATCCGGCGTAACGCCGGCGGTGAGATGAGGAG
GAGAGAGAGAGAAGGTGAATTTCCGACAAGAAGAGTGAGAAGAGAAAGAAGACTCATTGT
TTTGTTTTGTCAATCAATGGTCAAATTATGAGACGCGAAAAAGAGAGAGAGAGATGGTAA
ATCAGAGACCGTTGAAAGAGGATAGAATTGGACGGTGGAGATTAAGTCCCACCATTAAGT
ACCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCACA

Last Updated on 06.12.2018 16:11