Sequences from confirmation amplicon


Confirmation sequences for line 556E04 and hit At1g32130 are shown.
Line ID 556E04
Locus At1g32130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11560184
Primers used for PCR AY63 - 8474, show primer details
   
Sequence Name 11-K104808-0022-556-E04C-AY63
GenBank Accession LN491101 [GenBank]
Sequencing primer gene specific primer, AY63: 5'-TTGCTAGGTCTATCCCCAGTAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K104808-0022-556-E04C-AY63
GAATGTGAAGCGAGTAAAATAAAAAAAATAAAGGTTTCGGGAAATACTTCTTCACTGGTG
GTCTTCTATAGGGAACCCTATCTTCATCGAGATTTCTCATGTCCTCAAACCTAGTACTCT
TGTTGAAAATTGGACGAGACTGCATCATCAAGTATACAGTAAGTAACTGGCTGGAATTTG
ATTCGAGGGATTGGAGCTCTTGAAAAAGATGATTACCCATTTATCAACCAAATCCTTAGC
AAGTCGTCTGTTGGAATTAGTTTCCTCGTCGGACTTTGATAAGAACATAATGACCTGCCA
AAGCCAGAACAAATTTAAGTTCCTGAGTATAAATGTAAATCAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 27-K104809-0022-556-E04C-8409
GenBank Accession LN498373 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K104809-0022-556-E04C-8409
TCGGACATGAGCCATTTACAATTGAATATATCCTGATTTACATTTATACTCAGGAACTTA
AATTTGTTCTGGCTTTGGCAGGTCATTATGTTCTTATCAAAGTCCGACGAGGAAACTAAT
TCCAACAGACGACTTGCTAAGGATTTGGTTGATAAATGGGTAATCATCTTTTTCAAGAGC
TCCAATCCCTCGAATCAAATTCCAGCCAGTTACTTACTGTATACTTGATGATGCAGTCTC
GTCCAATTTTCAACAAGAGTACTAGGTTTGAGGACATGAGAAATCTCGATGAAGATAGGG
TTCCCTATAGAAGACCACCAGTGAAGAAGTATTTCCCGAAACCTTTATTTTTTTTATTTT
ACTCGCTTTCACATTTCATATACTGATTCATAGCTTCTCTTCTTGTTCTACTGGGGATAG
ACCTAGCA
Primers used for PCR AY62 - 8474, show primer details
   
Sequence Name 12-K104808-0022-556-E04C-AY62
GenBank Accession LN491566 [GenBank]
Sequencing primer gene specific primer, AY62: 5'-TGCCTGAGAGAGAATTTTGTTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K104808-0022-556-E04C-AY62
ATGATGAGAAGGTGTTAGGACTATGGATGATGATAATTTCATTGACGATACTGGTTTGGA
TCCTTCTGAGAGGTATGGAGGTGACGCTGGAGACCGTTCTCCAACTCATTATCCTCAGGT
ATGAGTAGTGTATCTTCAATTTTGATCTTTGAGTCTAGCTTTTGTTTGTTGATACAAAAT
GCTTTTGTTATGAATAAACAGGCAGAAGAGGGTGAGGATGAAGATGAGGTAAATAACCTT
TTCAAAATGGGGAAGAAAAAGAAAAAGACTGAAAGAAATCCTGCGGAAATTGCGCTTTTG
GTTGAGAATGTGATGGCTGAGCTTGAGGTTACTGCTGAGGAAGATGCAGAACTTAATAGA
CAGGGGAAGCCTGCTATCAACAAGCTTAAGAAACTTTCGCTTCTTACCGATGTACTTGGA
AAGTAAGTTGCTAACTTACTGTTATCTTATACTGTTTCTTTCTAACAATCTGTTTTGTAA
TACGTTTATTTTTCGTTATCCAGGAAGCAGCTTCAAACAGAATTCCTGGACCATGGAGTT
TTAACCCTGTTGAAGAATTGGCTTGAGCCTCTTCCAGATGGAAGCTTGCCAAATATAAAC
ATCCGTGCAGCTATTCTAAGAGTTCTTACTGATGTATGCTCTTGTTTTCCTGTTGAGGGT
TTTTCCGTGATTTTGAGCCTTTTTAGCTGCTTTACCAATTACTGAGTCTGTCTTGCAGTT
TCCGATTGACTTGGACCAGTATGATAGAAGGGAGCAATTGAAAAAGAGTGGGCTTGGAAA
GGTTAGGATACATTACCTGATTCAAAGTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAATGT
   
Sequence Name 28-K104809-0022-556-E04C-OriL
GenBank Accession LN498834 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K104809-0022-556-E04C-OriL
GCCTTTACCTTTGAATCAGGTAATGTATCCTAACCTTTCCAGCCCACTCTTTTTCAATTG
CTCCCTTCTATCATACTGGTCCAAGTCAATCGGAAACTGCAAGACAGACTCAGTAATTGG
TAAAGCAGCTAAAAAGGCTCAAAATCACGGAAAAACCCTCAACAGGAAAACAAGAGCATA
CATCAGTAAGAACTCTTAGAATAGCTGCACGGATGTTTATATTTGGCAAGCTTCCATCTG
GAAGAGGCTCAAGCCAATTCTTCAACAGGGTTAAAACTCCATGGTCCAGGAATTCTGTTT
GAAGCTGCTTCCTGGATAACGAAAAATAAACGTATTACAAAACAGATTGTTAGAAAGAAA
CAGTATAAGATAACAGTAAGTTAGCAACTTACTTTCCAAGTACATCGGTAAGAAGCGAAA
GTTTCTTAAGCTTGTTGATAGCAGGCTTCCCCTGTCTATTAAGTTCTGCATCTTCCTCAG
CAGTAACCTCAAGCTCAGCCATCACATTCTCAACCAAAAGCGCAATTTCCGCAGGATTTC
TTTCAGTCTTTTTCTTTTTCTTCCCCATTTTGAAAAGGTTATTTACCTCATCTTCATCCT
CACCCTCTTCTGCCTGTTTATTCATAACAAAAGCATTTTGTATCAACAAACAAAAGCTAG
ACTCAAAGATCAAAATTGAAGATACACTACTCATACCTGAGGATAATGAGTTGGAGAACG
GTCTCCAGCGTCACCTCCATACCTCTCAGAAGGATCCAAACCAGTATCGTCAATGAAATT
ATCATCATCCATAGTCCTAACACCTTCTTCATCATTCTACAACAGAAACGAAACACTTTT
ATAAGTCGAAAA

Last Updated on 06.12.2018 16:11