Sequences from confirmation amplicon


Confirmation sequences for line 557F12 and hit At3g20880 are shown.
Line ID 557F12
Locus At3g20880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7315167
Primers used for PCR MN08 - 8474, show primer details
   
Sequence Name 35-K028350-022-557-F12C-8409
GenBank Accession LN501703 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K028350-022-557-F12C-8409
TACAATTGAATATATGGTGAAAAAGAAAGAGTAATTACCAATTTTTTTCTACCATGGACT
TGAAGTTACTATGGCTTCTGACCATGATGATGAACATGGTGGCTTACAAAGGGGTAATCA
TCTTCATCACTTTTGGATCCCGACTCCTTCTCAGATTCTGATGGGTCCAACACAGTTCTC
TTGTCCTCTATGCTTCAAGACTTTCAACAGATACAACAACATGCAAGTAAGGATATATTC
ATTCAATAAACCTAACTCTAATTAGTTGCTATTTTCCATAAAATGATTTTCAAGTTTTGA
CTTTTTGTTCTTGAAAATAGATTAGTTTTCTTATTTGTATCTACATAATAATTCTTGTTA
TTTATTTATTTTTCAAATCTTCATCATGATAAAACCCCTAGTACACACTACGACTTTTCA
TAATAATTATTACTATTATAACTCGAGAAATAACTTTTAATTTGATGGTACAAG
   
Sequence Name 35-K028350-022-557-F12C-MN08
GenBank Accession LN501704 [GenBank]
Sequencing primer gene specific primer, MN08: 5'-CGTACAGCTGATGACTTGTACCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K028350-022-557-F12C-MN08
AAGAATTTGAAAAAATAAATAAATCAACAAGAAATTCATTATCGTAGATCACAAATAAGA
AAAACTAATCTATTTTCAAGAAACAAAAAGTCAAAACTTCGAAAATCATTTTATGGAAAA
TAGCAACTAATTAGAGTTAGGTTTATTCGAATGAATATATCCTTACTTCGCATGTTCGTT
CGTATCTGTTGAAAGTCTTGAAGCATAGAGGACAAGAGAACTGTGTTGGACCCATCAGAA
TCTGAGAAGGAGTCGGGATCCAAAAGTGATGAAGATGATTACCCCTTTGTAAGCCACCAT
GTTCATCATCATGGTCAGAAGCCATAGTAACTTCAAGTCCATGGTAGAAAAAAATTGGTA
ATTACTCTTTCTTTTTCTCCATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAAATCTTTATTTCGACGTGTCTA
Primers used for PCR MN09 - 8474, show primer details
   
Sequence Name 41-K028351-022-557-F12C-8409
GenBank Accession LN503952 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K028351-022-557-F12C-8409
CATTACAATTGAAGGACCATGATGGTCATCATCATGGTCAGAAGTCATTGGTGACCCTCT
TGGTGGTCGGTGGAATCAAGGACCACATCGCTGCTGCTGCTTCCACCATCACCGAGGTTT
GGTAACCCTAGATGAAGATCCACGGTTACATCATGACGATAACTGTCTTGGCATTGATCA
AGAAAGTTTGAATTAGAGGAAGTTTCTGTCGTCTCCATGAAATAGTACTCATGGTGGTTA
GCAGTAGGTTCTTGATGCTTATGAATGGGGCTTAAGCTAAGGAGAGGGAGGGCTTCTCTA
AGTGGAGGAGAGTCAGGTGAAGTCAAATTAGGGTTTTCGTTGATGTTTGGGTTTGTCGTC
TGGTGAGGATAATAAAAGCTGTCATGAGAGTTGTAGATGCGTGAGAGGGAAGTGGCATAG
TTGAAGCTATAGTTATAGTAGTCTACATATCCATTTCTGAAGATACAATCTGGATCAGCC
ATTAACTTTAGGAGAGAAGATAGTGAGAAAAAAAGAAGAAGAAGGCAAGATATCTCTAGA
TGATGGAGTTGTGT
   
Sequence Name 41-K028351-022-557-F12C-MN09
GenBank Accession LN503953 [GenBank]
Sequencing primer gene specific primer, MN09: 5'-CACTGGTTTCTTAACACAACTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K028351-022-557-F12C-MN09
TCCAGATTGTATCTTCAGAAATGGATATGTAGACTACTATAACTATAGCTTCAACTATGC
CACTTCCCTCTCACGCATCTACAACTCTCATGACAGCTTTTATTATCCTCACCAGACGAC
AAACCCAAACATCAACGAAAACCCTAATTTGACTTCACCTGACTCTCCTCCACTTAGAGA
AGCCCTCCCTCTCCTTAGCTTAAGCCCCATTCATAAGCATCAAGAACCTACTGCTAACCA
CCATGAGTACTATTTCATGGAGACGACAGAAACTTCCTCTAATTCAAACTTTCTTGATCA
ATGCCAAGACAGTTATCGTCATGATGTAACCGTGGATCTTCATCTAGGGTTACCAAACCT
CGGTGATGGTGGAAGCAGCAGCAGCGATGTGGTCCTTGATTCCACCGACCACCAAGAGGG
TCACCAATGACTTCTGACCATGATGATGACCATCATGGTCCTTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11