Sequences from confirmation amplicon


Confirmation sequences for line 561F07 and hit At1g18860 are shown.
Line ID 561F07
Locus At1g18860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6510365
Primers used for PCR IS52 - 8474, show primer details
   
Sequence Name 01-K027788-022-561-F07C-8409
GenBank Accession LN486435 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K027788-022-561-F07C-8409
AGCCATTTACATTTAGAATATAATTATCAGTTCAATTAGGACATGTAGATTTACCTGGTT
TTCTTACTGGACAAGAAGCTGCAATGGTGCAACGATAGTAAGCTCGGGGACATGGATTGC
CTTTAGCTATTTTCTGGCCATATTTCCTCCATTGACATCCGTCGTTCATCTACAGAGACA
ATATATAGCCCAAGAAATTAAAACACAAACTTTGATTCTGTTCTTGTGGAATATACTCTT
TGAGAAGTTTTTTTTTCTTATAAAAATAGAAGTGAGAAAAAAGGAAGGATTATACTGTTA
GCATTCATAGATGTATATACTAATTAAATTAAAGATATGAATAAAATGTACTAACCGTTG
GTGTCTCACATCTTGATCTCACCGAAACCCTAGTTTTCTTAACAAGGTTTTGAGGCAAAA
TCTCATCTTCATCTTCATGATCATCTCCATCATTCTTAAACCCAAAACTATTTTGTGATC
TGATCTTATTATTGTTACTAATCTCCAACGACATGGTTTCTTGATTATGATCAATCTCTA
ACTTCTCATTAGGATTACTCAAAGCCTTGTATTCAATCCCCATACTCAACCCTTGAATAC
TGCTTTTTTCATTATCATCATAATTTCTGTCACCTTCCGCTCT
   
Sequence Name 02-K027788-022-561-F07C-IS52
GenBank Accession LN486893 [GenBank]
Sequencing primer gene specific primer, IS52: 5'-AAATAAAGATGTTGAAGAAGCGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K027788-022-561-F07C-is52
ATGAAAAAGCGTATTCAAGGGTTGAGTATGGGGATTGAATACAAGGCTTTGAGTAATCCT
AATGAGAAGTTAGAGATTGATCATAATCAAGAAACCATGTCGTTGGAGATTAGTAACAAT
AATAAGATCAGATCACAAAATAGTTTTGGGTTTAAGAATGATGGAGATGATCATGAAGAT
GAAGATGAGATTTTGCCTCAAAACCTTGTTAAGAAAACTAGGGTTTCGGTGAGATCAAGA
TGTGAGACACCAACGGTTAGTACATTTTATTCATATCTTTAATTTAATTAGTATATACAT
CTATGAATGCTAACAGTATAATCCTTCCTTTTTTCTCACTTCTATTTTTATAAGAAAAAA
AAACTTCTCAAAGAGTATATTCCACAAGAACAGAATCAAAGTTTGTGTTTTAATTTCTTG
GGCTATATATTGTCTCTGTAGATGAACGACGGATGTCAATGGAGGAAATATGGCCAGAAA
ATAGCTAAAGGCAATCCATGTCCCCGAGCTTACTATCGTTGCACCATTGCAGCTTCTTGT
CCAGTAAGAAAACAGGTAAATCTACATGTCCTAATTGAACTGATAATTATATTCTAAATG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTAT
AGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11