Sequences from confirmation amplicon


Confirmation sequences for line 565B09 and hit At1g65880 are shown.
Line ID 565B09
Locus At1g65880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24510581
Primers used for PCR IW35 - 8474, show primer details
   
Sequence Name 18-K033212-022-565-B09C-8409
GenBank Accession LN494202 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K033212-022-565-B09C-8409
TTACACTTGGCCGCTTCTCTAATCTCTCTTAACATCTCCAAGAACGATGTGGTATGTCAC
ATTATTGGAGTTCAATATTTATAAATTTAAGCCCATCTGGATAAAACTATAGTTGTGATA
ACAAGATTTATTATGTTTATTTGTTTAATCATATTGTACCACTATCTTTCACTATCCTTA
CCTCAATTTTTCGCTGAAGAGGTTTAAGATTTGTTAGAGATAGTCCGTTTTGTGTAGGGT
ATAATTAAACAGATTAACATTCATTTATACAAAGTATGAGACATGCATGTGACTAACATT
GTGTATTTAGGTATCAGTTATGGCTCCAAACACACCCGCCTTGTATGAAATGCACTTTGC
CGTTCCCATGGCTGGAGCTGTACTTAACCCTATCAACACTCGTCTAGACGCAACATCCAT
TGCCGCAATCCTCCGCCACGCCAAGCCCAAGATCTTATTCCTAG
   
Sequence Name 26-K033212-022-565-B09C-IW35
GenBank Accession LN497816 [GenBank]
Sequencing primer gene specific primer, IW35: 5'-TCTAGGAATAAGATCTTGGGCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K033212-022-565-B09C-IW35
AGTGTTGATAGGGTTAAGTACAGCTCCAGCCATGGGAACGGCAAAGTGCATTTCATACAA
GGCGGGTGTGTTTGGAGCCATAACTGATACCTAAATACACAATGTTAGTCACATGCATGT
CTCATACTTTGTATAAATGAATGTTAATCTGTTTAATTATACCCTACACAAAACGGACTA
TCTCTAACAAATCTTAAACCTCTTCAGCGAAAAATTGAGGTAAGGATAGTGAAAGATAGT
GGTACAATATGATTAAACAAATAAACATAATAAATCTTGTTATCACAACTATAGTTTTAT
CCAGATGGGCTTAAATTTATAAATATTGAACTCCAATAATGTGACATACCACATCGTTCT
TGGAGATGTTAAGAGAGATTAGAGAAGCGGCCAAGTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAAAATGG

Last Updated on 06.12.2018 16:11