Sequences from confirmation amplicon


Confirmation sequences for line 565B11 and hit At3g09960 are shown.
Line ID 565B11
Locus At3g09960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3062467
Primers used for PCR PU08 - 8474, show primer details
   
Sequence Name 07-K026766-022-565-B11C-8409
GenBank Accession LN489112 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K026766-022-565-B11C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGCCATCTACTTTTGA
ATCTTATGGCGTTCCTCATGGATCTTCTGGTGACATTCTCCATAGATTCTTCTTTCTGGT
TATAGTTTATGATTAGACGTTTGTCTTTGTCGGAAACTGTAAATATAGGTCAAGTGATCG
ATCATGTAATTGGTGATCAGTCAAATAAGTAATGATTGGATCTTTGGTTAACCAAAAATA
TAATTGAATCTTTCTCGATTTGGAGTTTTCATGTTCTCATCTCTGTACTAGTGAAATTAT
GAAATTTTATAACTGTTTTGAATCTATTGGTATTGGTTTTGAAACTTGTTTGTTTTCTTT
TCCTTGATAGATTTGATAAAGGCAGTACCTGAGAGTCACAAGAAATTCTTGACCAATATG
GTTTGGGTACATAAAGAGGTAAGGGCTTTTCTCCTACCATGTGACGTTTCTTA
   
Sequence Name 08-K026766-022-565-B11C-PU08
GenBank Accession LN489575 [GenBank]
Sequencing primer gene specific primer, PU08: 5'-CATTTGTTCCTAAGAAACGTCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K026766-022-565-B11C-pu8
GAGAAAAGCCCTTACCTCTTTATGTACCCAAACCATATTGGTCAAGAATTTCTTGTGACT
CTCAGGTACTGCCTTTATCAAATCTATCAAGGAAAAGAAAACAAACAAGTTTCAAAACCA
ATACCAATAGATTCAAAACAGTTATAAAATTTCATAATTTCACTAGTACAGAGATGAGAA
CATGAAAACTCCAAATCGAGAAAGATTCAATTATATTTTTGGTTAACCAAAGATCCAATC
ATTACTTATTTGACTGATCACCAATTACATGATCGATCACTTGACCTATATTTACAGTTT
CCGACAAAGACAAACGTCTAATCATAAACTATAACCAGAAAGAAGAATCAATGGAGAATG
TCACCAGAAGATCCATGAGGAACGCCATAAGATTCAAAAGTAGATGGCCAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAGAGTAATTCCAATTTTTT

Last Updated on 06.12.2018 16:11