Sequences from confirmation amplicon


Confirmation sequences for line 567F10 and hit At1g20980 are shown.
Line ID 567F10
Locus At1g20980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7326247
Primers used for PCR NJ37 - 8474, show primer details
   
Sequence Name 63-K036619-022-567-F10C-8409
GenBank Accession LN510927 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K036619-022-567-F10C-8409
CCATTTAAATTGAAATATCCTGATGAAAGATCCATCATAATTAATGATAACTTAGTCTTC
TCGCTGTCTTTGTTACCAAACCCACCTTGAGATAATATCGCTAGTGCATCAGGTGAAGAT
GAACCTAATGTCGTCGAAAGAACAGCAAGCAAGTCCATGGTAGAAGGAGAAGCCCCATTC
ATATCATTTTGTGGGTTCACCGTCGGGTGATCCATATTTTTCCTGGCTAAACTTCCAATA
TTGTTCAACTTAGAGACAAGATCCATAGGCAAAGGCAATGCATTTATCTTGTTAAGTATC
TGAAGAAGCTGCTCTCTATCAGGCACAGCTGGAGATCCCACTGGGGGCTTTACCGCATTC
TTACCTGCAGATCACACACAACAAAATCTGTTAATAAAACAAGAACATCAAAGCAGAGTT
CCCAGACTAAAATACTTCTACAAGTCGCAAAACTAGTGTACCTTGTGCACAAGCTAAGGC
GGTTAACAAAGCCATAAATCCCATATTGG
   
Sequence Name 64-K036619-022-567-F10C-NJ37
GenBank Accession LN511143 [GenBank]
Sequencing primer gene specific primer, NJ37: 5'-CCAATATGGATCTTATGGCTTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K036619-022-567-F10C-NJ37
TAGTTTTGCGACTTGTAGAAGTATTTTAGTCTGGGAACTCTGCTTTGATGTTCTTGTTTT
ATTAACAGATTTTGTTGTGTGTGATCTGCAGGTAAGAATGCGGTAAAGCCCCCAGTGGGA
TCTCCAGCTGTGCCTGATAGAGAGCAGCTTCTTCAGATACTTAACAAGATAAATGCATTG
CCTTTGCCTATGGATCTTGTCTCTAAGTTGAACAATATTGGAAGTTTAGCCAGGAAAAAT
ATGGATCACCCGACGGTGAACCCACAAAATGATATGAATGGGGCTTCTCCTTCTACCATG
GACTTGCTTGCTGTTCTTTCGACGACATTAGGTTCATCTTCACCTGATGCACTAGCGATA
TTATCTCAAGGTGGGTTTGGTAACAAAGACAGCGAGAAGACTAAGTTATCATTAATTATG
ATGGATCTTTCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAAATGGG

Last Updated on 06.12.2018 16:11