Sequences from confirmation amplicon


Confirmation sequences for line 576G03 and hit At1g08160 are shown.
Line ID 576G03
Locus At1g08160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2559910
Primers used for PCR NG32 - 8474, show primer details
   
Sequence Name 04-K102362-0022-576-G03C-8409
GenBank Accession LN487863 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K102362-0022-576-G03C-8409
TTACAATTGAATATATCCTGGAAGAGACTTAAGGGCTGAACTTAAGTCTAGTAGCTTGTG
TAGACTTAAGGACTTAAGGACATCGCAGCTTGTGTCCCACAATGTGGCACTGTCTAAGTT
CAATGCCAGGGACTTAAGGGCTGAAAAATCAAAAGGGACGATCGAAATGGAAGTGTATAT
AACGGCTAGAGTGAGCTACAAGACGTGGATTTTCCGGTCGAGAAGACGTACGTTGAAGGC
TGTGTGTACGCCGGTAATGATCAACGTTACATCAAGCTCGTTGGATGGATTCCAGAGAGT
GTTATGCAAAACTCGTCTTTAGTTTGATTTATCCTCATCTTGAATTGGTTTCATTTGTGT
GCAAAATTTGCTATATGTGACATTGATTGATTGTGTGTGTGTTTTTCCTTTTTAAATTTC
CTCTTTGGTTTTGTTCTTTTCTG
   
Sequence Name 04-K102363-0022-576-G03C-NG32
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, NG32: 5'-CAGAAAAGAACAAAACCAAAGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K102363-0022-576-G03C-NG32
GACCGCTCCCATCCAAGATCTGAAAAGATACCGACTTTCTGTACAAACTTTCTGCATAGA
CTTAAATCCTCCTTAAGTGATACTCTCAGATTTTAGTAAAGAGCTCAATATTTCGATAGT
CTACTGCATATACTATACAGACAGAACCAATTCTGACTTTAAAGAGTCTGAAGAAACTGA
TCTTTAAACAACACAGTGCCTCCGCTTTCGGATCAGAACATTCTAGTCAAAATTCACATT
CCTAATGCCAAATAGTAATCTCCTTTAGATCCCATTAGCTCTAAATTAACTGGAATAAAT
AACAAATAAGTAAGAAA

Last Updated on 06.12.2018 16:11