Sequences from confirmation amplicon


Confirmation sequences for line 580A05 and hit At5g24520 are shown.
Line ID 580A05
Locus At5g24520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8372503
Primers used for PCR ZZ85 - 8474, show primer details
   
Sequence Name 15-K024135-022-580-A05C-ZZ85
GenBank Accession LN492792 [GenBank]
Sequencing primer gene specific primer, ZZ85: 5'-GAGAGAGAGCAACAATACGAGACA-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K024135-022-580-A05C-ZZ85
TACCGCCGGCGCAACTGCTTCCAAATATTGACAAACAAATTTGAATCTGGATCTTCTCTA
TTCGTGAACAAGGAGATAGAAGCTACGATGAATGCATGGAAGCTTGGTTTGCTTTAATAT
AAACACTAAAGGGGAGTAGAACTTTCTTGAAAAATTGTATGCAAATTATTTACCGAATGT
TAAAAGCTTTTTTCGAATAAATTTTACATTTTCTTAATAATAATAATAAAAAAGGATTGT
TGATTATCTTAATCACAAACAATTTATTTTAGCTGAATTAGACAATTGTTAGTAAAATGA
TTAGAGTGTCACATATTAATGTTGTTAGTGTTTCATGTCATCCTAGTGATCCAATAATTA
GGCCATTCTATAGCTCTTAACGTTAAAATAAAAGGCCCATTATCTGAATATACAGAAGCC
CATTATCAATAGATACATTAAAAGATACTGATTAATCCAGAGGGTTTATATCTACGCCGT
CTCCATTGATTATTTCTCCGTCTCTTGAAAAATCCGACTGACACTGACCTCAAAACTCTC
CTCTCACTTTCGTCGTGAAGAAGCCAAATCTCGAATCGAATCAGCACCACACATTTCCAT
GGATAATTCAGCTCCAGATTCGTTATCCAGATCGGAAACCGCCGTCACATACGACTCACC
ATATCCACTCTACGCCATGGCTTTCTCTTCTCTCCGCTCATCCTCCGGT
   
Sequence Name 16-K024135-022-580-A05C-8409
GenBank Accession LN493261 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K024135-022-580-A05C-8409
GTAGATTTCCCGGACATGAAGCCATAAGGATGCTCGAAGGAGAGATTCGGGAGAGGCTTA
ACGGTCATTGAATCGGAATCGAAAGAGAGAATGTCGATGCGGTTGTTGTAATCTTCGAGG
AAGCTTCCGACGGCGATTCTGTGACCGGAGGATGAGCGGAGAGAAGAGAAAGCCATGGCG
TAGAGTGGATATGGTGAGTCGTATGTGACGGCGGTTTCCGATCTGGATAACGAATCTGGA
GCTGAATTATCCATGGAAATGTGTGGTGCTGATTCGATTCGAGATTTGGCTTCTTCACGA
CGAAAGTGAGAGGAGAGTTTTGAGGTCAGTGTCAGTCGGATTTTTCAAGAGACGGAGAAA
TAATCAATGGAGACGGCGTAGATATAAACCCTCTGGATTAATCAGTATCTTTTAATGTAT
CTATTGATAATGGGCTTCTGTATATTCAGATAATGGGCCTTTTATTTTAACGTTAAGAGC
TATAGAATGGCCTAATTATTGGATCACTAGGATGACATGAAACACTAACAACATTAATAT
GTGACACTCTAATCATTTTACTAACAATTGTCTAATTCAGCTAAAATAAATTGTTTGTGA
TTAAGATAATCAACAATCCNTTTTTTATTATTATTATTAAGAAAATGTAAAATTTATTCG
AAAAAAGCTTTTAACATTCGGTAAATAATTTGCATACAATTTTTCAAGAAAGTTCTACTC
CCCTTTAGTGTTTATATTAAAGCAAACCAAG

Last Updated on 06.12.2018 16:11