Sequences from confirmation amplicon


Confirmation sequences for line 584D09 and hit At1g49770 are shown.
Line ID 584D09
Locus At1g49770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18425200
Primers used for PCR NB48 - 8474, show primer details
   
Sequence Name 55-K101327-0022-584-D09C-8409
GenBank Accession LN508754 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K101327-0022-584-D09C-8409
TTACATTGAAATATCCTGTAGATCTAATAAAATTGATGAGTTTATAACCTGTCGTTTCAC
CAAAAATAAGTCATTCTTTTGCATTTGGATAAGCCTAGACCATTCTCTGAATGCAAATGT
TATTTTCAGGATGAAATTGGATTTTTTTTAGTTATATGTATATGCATATCTGTAATTTTA
TATGATTGGTAATTCATACAGATAATCGTGTTATAAACACACAATTTTCGCATATAAATT
ACCGGTTAAAAACGATGGTAATTAATATATGAATTTCATGCTAAATAAGAAAAAATCCTT
CAATTAAAGCATACACTCTTATTATTGAATCATACCTTAGGAGGAAGTTGGGGAAGCAAA
GCATGTAATTTAGAAAACATGTCCCTCATCTTCTTTCTCCTTTCTCTTTCGGTCCATATA
TGTATTTCATGATCAGGTGATTCTTCTTCATGGTTTTTATCATTTCTTTTCGTTCTCTTT
TTTCCCTTTTTAACCGTCGTAT
   
Sequence Name 55-K101333-0022-584-D09C-NB48
GenBank Accession LN508755 [GenBank]
Sequencing primer gene specific primer, NB48: 5'-AGATCCAGACTACGACGGTTAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K101333-0022-584-D09C-NB48
AACCATGAAGAAGATCACCTGATCATGAAATACATATATGGACCGTAAGAGAAAGGAGAA
AGAAGATGAGGGACATGTTTTCTAAATTACATGCTTTGCTTCCCCAACTTCCTCCTAAGG
TATGATTCAATAATAAGAGTGTATGCTTTAATTGAAGGATTTTTTCTTATTTAGCATGAA
ATTCATATATTAATTACCATCGTTTTTAACCGGTAATTTATATGCGAAAATTGTGTGTTT
ATAACACGATTATCTGTATGAATTACCAATCATATAAAATTACAGATATGCATATACATA
TAACTAAAAAAAATCCAATTTCATCCTGAAAATAACATTTGCATTCAGAGAATGGTCTAG
GCTTATCCAAATGCAAAAGAATGACTTATTTTTGGTGAAACGACNGGTTATAAACTCATC
AATTTTATTTAGATCTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCNAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11