Sequences from confirmation amplicon


Confirmation sequences for line 585B07 and hit At5g49770 are shown.
Line ID 585B07
Locus At5g49770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20226878
Primers used for PCR EU08 - 8474, show primer details
   
Sequence Name 22-K103311x-0022-585-B07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K103311x-0022-585-B07C-8409
AAGCCATTTACAATTGAATATATCCTCGTCCGTCAATTGATTCGTCATGTAGTACTCAGG
ATCAAGATAGCCCTAATTAATTCCAGAGATATTGTTAGTTTTTATAGTTAATGGTTTCAT
ATATTTTATGATAGATTCTTTTTTACTCACCATGGTTCCTTTCACTTGTGTTGTGACATG
AGCTTTTTCAGGGTCCCCCACAAGTTTGGAGAGACCGAAATCAGCAACCTTTGCGGTTAG
ATGTTCATCAAGTAATATGTTATTTGATTTGACGTCTCTATGTATAATTGGAGGATCAGC
AAGCTCATGAAGATAAGCTAGACCCTTCCCTGAGCCAAGTGCTATTTTGAGCCTTCTTGT
CCAATCCAGTTTAACCCCATTCTTCCCTACATTTGTTTTTCAAGTTCAGCACCAAAGAAC
TAACAAAGCAAGATATATAGAGATTAAATATATACCTGATAGACCGTCTCTAAGGGACCC
ATTAGGTATGTACTCGTATACGAGCATTTGTTCTTTTTGATCAAAACAGAAGCCCAAAAG
CTTAACAACATTTTTATGATGGACCCTTGAAAGAAGCTCAATCTCGGTCTTAAACTCGAA
CGCTCCTTGCATGGATCCTTGTTGAGCTCTTTTGATTGCTATGACTTGCCCATTGGGAAG
GGTACCTTTGTAGACCTGTTTCCACCATC
   
Sequence Name 17-K103312x-0022-585-B07C-EU08
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, EU08: 5'-TGATGGTGAAACAGGTCTACAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K103312x-0022-585-B07C-EU08
AGTCATAGCAATCAAAAGAGCTCAACAAGGATCCATGCAAGGAGCGTTCGAGTTTAAGAC
CGAGATTGAGCTTCTTTCAAGGGTCCATCATAAAAATGTTGTTAAGCTTTTGGGCTTCTG
TTTTGATCAAAAAGAACAAATGCTCGTATACGAGTACATACCTAATGGGTCCCTTAGAGA
CGGTCTATCAGGTATATATTTAATCTCTATATATCTTGCTTTGTTAGTTCTTTGGTGCTG
AACTTGAAAAACAAATGTAGGGAAGAATGGGGTTAAACTGGATTGGACAAGAAGGCTCAA
AATAGCACTTGGCTCAGGGAAGGGTCTAGCTTATCTTCATGAGCTTGCTGATCCTCCAAT
TATACATAGAGACGTCAAATCAAATAACATATTACTTGATGAACATCTAACCGCAAAGGT
TGCTGATTTCGGTCTCTCCAAACTTGTGGGGGACCCTGAAAAAGCTCATGTCACAACACA
AGTGAAAGGAACCATGGTGAGTAAAAAAGAATCTATCATAAAATATATGAAACCATTAAC
TATAAAAACTAACAATATCTCTGGAATTAATTAGGGCTATCTTGATCCTGAGTACTACAT
GACGAATCAATTGACGGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATNTCCGCA
Primers used for PCR NK74 - 3144, show primer details
   
Sequence Name 42-K106973-0022-585-B07C-3144
GenBank Accession LN504476 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K106973-0022-585-B07C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGGGTTTGGTGT
GGTGATGCTTGAGTTATTAACCGGTAAAAGTCCGATAGATAGAGGCAGCTATGTTGTGAA
AGAGGTAAAGAAGAAGATGGATAAATCGAGGAACTTGTATGACTTACAAGAATTGTTGGA
CACGACAATTATCCAAAACAGCGGGAATTTGAAAGGGTTCGAGAAGTATGTAGATGTGGC
TCTACAATGCGTGGAGCCAGAGGGAGTTAATAGGCCAACAATGAGTGAGGTGGTGCAAGA
ACTTGAGAGCATATTGCGGCTTGTTGGATTAAACCCTAACGCCGATTCAGCAACATACGA
GGAAGCATCTGGTGATCCTTATGGTAGGGATTCGTTTGAGTACACTGGGGTTTTCCCAAC
TCCAAAACCCAAACCATTTAATTGA
   
Sequence Name 58-K106974-0022-585-B07C-NK74
GenBank Accession LN509716 [GenBank]
Sequencing primer gene specific primer, NK74: 5'-ATCAATTATTGCGTTAGGGTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K106974-0022-585-B07C-NK74
AATCCCTACCATAAGGATCACCAGATGCTTCCTCGTATGTTGCTGAATCGGCGTTAGGGT
TTAATCCAACAAGCCGCAATATGCTCTCAAGTTCTTGCACCACCTCACTCATTGTTGGCC
TATTAACTCCCTCTGGCTCCACGCATTGTAGAGCCACATCTACATACTTCTCGAACCCTT
TCAAATTCCCGCTGTTTTGGATAATTGTCGTGTCCAACAATTCTTGTAAGTCATACAAGT
TCCTCGATTTATCCATCTTCTTCTTTACCTCTTTCACAACATAGCTGCCTCTATCTATCG
GACTTTTACCGGTTAATAACTCAAGCATCACCACACCAAACCCAAACACTGATAGTTTAA
ACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGA
ACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTAC
GTCAGTGGAGATATCAAACCAATCCA

Last Updated on 06.12.2018 16:11