Sequences from confirmation amplicon


Confirmation sequences for line 588D06 and hit At2g04890 are shown.
Line ID 588D06
Locus At2g04890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1720593
Primers used for PCR TA25 - 8474, show primer details
   
Sequence Name 31-K030141-022-588-D06C-8409
GenBank Accession LN500035 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K030141-022-588-D06C-8409
GCCATTTACAATTGAATATATCCTGAGACCAGATTCGGTCCATCCAACCAAGGTACAGAG
CACCATCTCTTTCTTCAATCGCATACCCGTTGCTGTAATCTCTCAAGAGGGCTCTAATGG
TGGCTGAAATGATTGAGCTCAAGGGGTATGGCTCAAAACCCGCCATGGAAAACCTTGACT
TCCATTTCCCGAGAAGCTCGTGTCTTTCGATCCTCTCGGCTCCTTCACAAGCTATGATGT
TGACGACATCCCTTGCCATGCAGTGCTGCTCGATATTGATCCTTTCCTTGTGATTTCTCG
GAAGCATAACATCGATAGATTCGAACATTGCCGTGTAATAACTTAATGTCTCAAGGAACC
TAGGAAGGAAAGGGGAAGTGTTCGTGTTGCATTCTTGTTCCACAAGAGTGACTACTTTAG
GTGATAGACTCTTCACCATCCTCAGCAACCGGTCCCTGTGGTTTTCCATGCTTACACTCT
CATCTGGCAAATGATGCAGCATGTAAGCAAAGTTCACTCCAAGGGCTTCGCCATCTCGGA
CATCAAGATTTTCCACTT
   
Sequence Name 31-K030141-022-588-D06C-TA25
GenBank Accession LN500036 [GenBank]
Sequencing primer gene specific primer, TA25: 5'-CAAGTTGTGAAGTTGAAGTGGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K030141-022-588-D06C-TA25
CTTTGCTTACTGCTGCATCATTTGCCAGATGAGAGTGTAAGCATGGAAAACCACAGGGAC
CGGTTGCTGAGGATGGTGAAGAGTCTATCACCTAAAGTAGTCACTCTTGTGGAACAAGAA
TGCAACACGAACACTTCCCCTTTCCTTCCTAGGTTCCTTGAGACATTAAGTTATTACACG
GCAATGTTCGAATCTATCGATGTTATGCTTCCGAGAAATCACAAGGAAAGGATCAATATC
GAGCAGCACTGCATGGCAAGGGATGTCGTCAACATCATAGCTTGTGAAGGAGCCGAGAGG
ATCGAAAGACACGAGCTTCTCGGGAAATGGAAGTCAAGGTTTTCCATGGCGGGTTTTGAG
CCATACCCCTTGAGCTCAATCATTTCAGCCACCATTAGAGCCCTCTTGAGAGATTACAGC
AACGGGTATGCGATTGAAGAAAGAGATGGTGCTCTGTACCTTGGTTGGATGGACCGAATC
TTGGTCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
ATCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11