Sequences from confirmation amplicon


Confirmation sequences for line 589H11 and hit At4g05540 are shown.
Line ID 589H11
Locus At4g05540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2820635
Primers used for PCR KY03 - 8474, show primer details
   
Sequence Name 07-K036056-022-589-H11C-8409
GenBank Accession LN489178 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K036056-022-589-H11C-8409
CAGCCAAAATTGCAGATTCTTGAGAAGAGTTCAAATTCGCTGATCGTATAATATCTAACA
CATGATTCCCCCAATTCCTAGAAGAAACAGATTGCTCTGTATCCTGAAATATTTACAAGA
AAAACCACAGTTACTCCACATTAGACGCTAAAGCCAGATCATTTCATGTTTACTTACCGC
GGTATTTGCTTGAAGGACACTCTTGATGAGACTCAAGTTTCCATCTCCGTTGTGCAAAGC
CTTCCAGATACGAGTGTTTGTGGTTAAGTATATAAGAAAAAAACCCGAACGAAACGAATA
TTGCTCATCAATGGATATAGATTTAGAGGAATGAACAGAGAAATGTAGCTCATCTCCAAC
AGAGAACACATACGCAAGAATCAGTGGGTTTAGNTCATCC
   
Sequence Name 08-K036056-022-589-H11C-KY03
GenBank Accession LN489646 [GenBank]
Sequencing primer gene specific primer, KY03: 5'-GTGGATGACTTAAACCCACTGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K036056-022-589-H11C-KY03
ATTTCACTGTTCATTCCACTAAATCTATATCCATTGATGAGCAATATTCGTTTCGTTCGG
GTTTTTTTCTTATATACTTAACCACAAACACTCGTATCTGGAAGGCTTTGCACAACGGAC
ATGGAAACTTGAGTCTCATCAGGAGTGTCCTTCAAGCAAATACCGCGGTAGGTAAACATG
AGATGATCTGGCTTTAGCGTCTAAGGTGGAGTAACTGTGGTTTTTCTTGTAAATATTTCA
GGATACAGAGCAATCTGTTTCTTCTAGGAATTGGGGGAATCATGTGTTAGATATTATACG
AGCAGCGAATTTGAACTCTTCTCAAGAATCTGCAGTTTTGGCTTGCCTTGAGACAAGACA
AATCTACATGGATCGGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAATTATAAAATGAAAGTA
CATTTTGATAAAGCGACGAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAA
TTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAA

Last Updated on 06.12.2018 16:11