Sequences from confirmation amplicon


Confirmation sequences for line 592B03 and hit At3g27080 are shown.
Line ID 592B03
Locus At3g27080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9985730
Primers used for PCR RL01 - 8474, show primer details
   
Sequence Name 24-K027845-022-592-B03C-8409
GenBank Accession LN496860 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K027845-022-592-B03C-8409
AATTGAATATATCCTGCAGCAACCAGATAATACACACTACCTGAAATCACTCGAAATGAC
GGCCAAGGTACTTCTTTCTATAAACCATCAACCAATTTGTGTTCATATTCATTTTGCCTC
TAAACCCAATCTCAAAGTTTTATTCTTTGATCAAATTTCTTCTCCTTTGTGCTGAGTACA
GGCTCCACAGCTGCACGCAGAAGCTTACAAACAAGGCTTAGGCTCACAACCAATGGGTCG
CGTTGAAGCTCCAGCACCGCCGAGCTCAAAGGTTCTCTCTTTTTTATTTATGTTCATTCT
CATCATCACATTTCATTTTCATTGCTTGATGTAATCGACTTGTAGATAATATAAGAAGAT
GTGTTTGAACCTCATGATCATATTTTGATGTATTGTTCCAAACACAGGCAGTGAAGAATA
AGAAAAGTAGTGATGCCAAGTATGATGCTATGGGTTGGGTGATTCTAGCCATTGGTGTTG
TTGCTTGGATCAGTTTCGCGAAAGCTAATGTGCCTGTCTCTCCTCCTCGTTAAGTAGACT
CGTTAGGAGACTTTGATGAAGTTTTTCAATTTTTGAGGTTTTGACAGTTGGAGCTTGTTG
TGTAAGATTTTTAGTTGTACTACGAGTACTTTATTATTGAAAGAAGATAAATGTCTTTTT
ACTAGAGCTTGTTCTTCACG
   
Sequence Name 25-K027845-022-592-B03C-RL01
GenBank Accession LN497299 [GenBank]
Sequencing primer gene specific primer, RL01: 5'-GTTTAACATGAACGCTGAAGAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K027845-022-592-B03C-RL01
CTTCTTTCATAATAAAGTACTCGTAGTACAACTAAAAATCTTACACAACAAGCTCCAACT
GTCAAAACCTCAAAAATTGAAAAACTTCATCAAAGTCTCCTAACGAGTCTACTTAACGAG
GAGGAGAGACAGGCACATTAGCTTTCGCGAAACTGATCCAAGCAACAACACCAATGGCTA
GAATCACCCAACCCATAGCATCATACTTGGCATCACTACTTTTCTTATTCTTCACTGCCT
GTGTTTGGAACAATACATCAAAATATGATCATGAGGTTCAAACACATCTTCTTATATTAT
CTACAAGTCGATTACATCAAGCAATGAAAATGAAATGTGATGATGAGAATGAACATAAAT
AAAAAAGAGAGAACCTTTGAGCTCGGCGGTGCTGGAGCTTCAACGCGACCCATTGGTTGT
GAGCCTAAGCCTTGTTTGTAAGCTTCTGCGTGCAGCTGTGGAGCCTGTACTCAGCACAAA
GGAGAAGAAATTTGATCAAAGAATAAAACTTTGAGATTGGGTTTAGAGGCAAAATGAATA
TGAACACAAATTGGTTGATGGTTTATAGAAAGAAGTACCTTGGCCGTCATTTCGAGTGAT
TTCAGGTAGTGTGTATTATCTGGTTGCTGCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTT

Last Updated on 06.12.2018 16:11