Sequences from confirmation amplicon


Confirmation sequences for line 592D11 and hit At5g35770 are shown.
Line ID 592D11
Locus At5g35770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:13938182
Primers used for PCR BD57 - 8474, show primer details
   
Sequence Name 20-K028348-022-592-D11C-8409
GenBank Accession LN495075 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K028348-022-592-D11C-8409
ATTGAATAATAGTAGATGTTTCTCTGTCTCTCTCATCCCTCTCCAACAATCCGTGGACCT
ACATTCTCTACATTTACGTTTATATTGTTATTTATTGTACATCTTTATTCCTCATTAAAC
GCAAAGTAATGTATTTGACTTCGTACTCTAGCTCTATAGTACAACCTAAAATCAAATATT
TCGAATTCTATACGAAAAGAAAAGATATCTTGTCAAACCAAAGGTTTAATGTCTATTAGT
CAGATAATCATTATAAAAAAAGAATAATCTAGGCACTAGGGCACGAAATGTAACATAAAA
TAGATTAGTACAACAAATAACATATGATGGAATGGANAAATCCTA
   
Sequence Name 20-K028348-022-592-D11C-BD57
GenBank Accession LN495076 [GenBank]
Sequencing primer gene specific primer, BD57: 5'-AATAGGATTTGCCATTCCATCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K028348-022-592-D11C-BD57
AACAAGATATCTTTTTCTTTTCCGTAATAGAAATTCGAAATCATTCGATTTCTAGGTTCG
TACTAATAGAGCTAGAGTACGAAGTCAAATACATTACTTCGCGTTTAATGAGGAAATAAA
GATCGTACAATAAATAACAATATAAACGTAAATCGTAGAGAATGTAGGTCCACGGATTCG
TTGGAGTGGGATGAGAGAGACAGAGAAACATCTACTATATATTCAATTCGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11