Sequences from confirmation amplicon


Confirmation sequences for line 595D12 and hit At3g12100 are shown.
Line ID 595D12
Locus At3g12100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3855937
Primers used for PCR GG28 - 8474, show primer details
   
Sequence Name 96-K100901-0022-595-D12C-8409
GenBank Accession LN515377 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>96-K100901-0022-595-D12C-8409
CGGACTGAGCCTTTACAATTGAATATATCCTGTCACTAAAACATACCAATATTGACACGA
GCATACCTAGTAGGTTCACCAGCAGATTTGTTACCGCTGATAACCGACGATGTAATTTCC
GACGAGAAATAACCGACGATGATAAACATACCAATATTGACACGAGCATAATTCCGGAAA
AACCAAACACCTAGTAGGTTCACCAGCAGATTTGTTACCGCTGATACAATCAAATAATGC
CTACATAAAGGAAATCTCAGTAAATCACACTACTGAAAGGAACAAGAGGGAACAAGTACT
GTAAACTTAGTAAGTGCTAGAGATATATATAGAAACTCCTAGGCATGTCATTTGTGAAAA
TATCAACTTACTTATGCTCTGATTCATCTTGTATAAATGCATGAAGAGCTTCCACAGCTA
AGGAGAATGACATGAACATAAGGAACAGCTGTCTACACATATGTCAAATATTAAACAAAT
CAAAGTATTAACCAAAAAGATAAACTACAGAGACAATGAAAGGCAAAACGAAAAGATAGA
TTGTATGGAAGTCCAAATCTTTTTAACGAGCTGTAAAATACTCTATTCTATCAGCAACAG
TAAAGGAAAGAGTTTTAACACTGAGTCAAGCCATCTACACTAA
   
Sequence Name 96-K100907-0022-595-D12C-GG28
GenBank Accession LN515378 [GenBank]
Sequencing primer gene specific primer, GG28: 5'-TTCCAGCTTTAGTGTAGATGGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>96-K100907-0022-595-D12C-GG28
TGATAGAATAGAGTATTTTACAGCTCGTTAAAAAGATTTGGACTTCCATACAATCTATCT
TTTCGTTTTGCCTTTCATTGTCTCTGTAGTTTATCTTTTTGGTTAATACTTTGATTTGTT
TAATATTTGACATATGTGTAGACAGCTGTTCCTTATGTTCATGTCATTCTCCTTAGCTGT
GGAAGCTCTTCATGCATTTATACAAGATGAATCAGAGCATAAGTAAGTTGATATTTTCAC
AAATGACATGCCTAGGAGTTTCTATATATATCTCTAGCACTTACTAAGTTTACAGTACTT
GTTCCCTCTTGTTCCTTTCAGTAGTGTGATTTACTGAGATTTCCTTTATGTAGGCATTAT
TTGATTGTATCAGCGGTAACAAATCTGCTGGTGAACCTACTAGGTGTTTGGTTTTTCCGG
AATTATGCTCGTGTCAATATTGGTATGTTTATCATCGTCGGTTATTTCTCGTCGGAAATT
ACATCGTCGGTTATCAGCGGTAACAAATCTGCTGGTGAACCTACTAGGTATGCTCGTGTC
AATATTGGTATGTTTTTAGTGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAG

Last Updated on 06.12.2018 16:11