Sequences from confirmation amplicon


Confirmation sequences for line 596B04 and hit At2g05715 are shown.
Line ID 596B04
Locus At2g05715 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:2146820
Primers used for PCR ME12 - 8474, show primer details
   
Sequence Name 79-K104224-0022-596-B04C-ME12
GenBank Accession LN513823 [GenBank]
Sequencing primer gene specific primer, ME12: 5'-AATTGGCATACTTTAACCATGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K104224-0022-596-B04C-ME12
CTTGAGCAGCAATAGTGAAGGAAGCAATACACAAGTTATGGAGCAAAATAAAAGAGTGTT
TGTTTCTGTTTTAAAATGTCTTTTTTAAAAGGCAGATAAGTCTGTCTAGGATTAGGTTAA
AAGGTGAGTGATCTCATGCAATATATGAGTTTCAGTGGGTTATTCTTACACGCTTAGGCT
CCCATGTTTCGCAACTTGCAAGAGTTGAATATTATTGTTTTTGAAAAGGAATAAGAGAGC
CTTCTTGCATATTCCCATTACTGACTCCCAAAGACCTCGCAATCTGTTGCATTTCACAAG
CGTATTCAGCAGCATCTATACCAATTCTCAGATCATGTTCAAGAACACCTGCTCTATTCA
AATCTACTTGCACCATTAGAGTAACCAACTAATACATAAGAAACACAAACATCTTGCAGC
TGAAGCCTAAAACCAATACAAGAATCTGATAAAACCAAACTGAAACTTGTCATAAATTCC
ATATGAACCTGAGAGTAATCTCCTGTGTCTGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATTCC
   
Sequence Name 39-K104225-0022-596-B04C-8409
GenBank Accession LN503340 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K104225-0022-596-B04C-8409
TGGAATTATGACAAGTTTCAGTTTGGTTTTATCAGATTCTTGTATTGGTTTTAGGCTTCA
GCTGCAAGATGTTTGTGTTTCTTATGTATTAGTTGGTTACTCTAATGGTGCAAGTAGATT
TGAATAGAGCAGGTGTTCTTGAACATGATCTGAGAATTGGTATAGATGCTGCTGAATACG
CTTGTGAAATGCAACAGATTGCGAGGTCTTTGGGAGTCAGTAATGGGAATATGCAAGAAG
GCTCTCTTATTCCTTTTCAAAAACAATAATATTCAACTCTTGCAAGTTGCGAAACATGGG
AGCCTAAGCGTGTAAGAATAACCCACTGAAACTCATATATTGCATGAGATCACTCACCTT
TTAACCTAATCCTAGACAGACTTATCTGCCTTTTAAAAAAGACATTTTAAAACAGAAACA
AACACTCTTTTATTTTGCTCCATAACTTGTGTATTGCTTCCTTCACTATTGCTTGCTCAA
GTTTCTGATAACATATGGAAGTATGCCTCCNTGGTT
Primers used for PCR NJ50 - 8474, show primer details
   
Sequence Name 80-K104224-0022-596-B04C-NJ50
GenBank Accession LN513948 [GenBank]
Sequencing primer gene specific primer, NJ50: 5'-AAAGGTGAGTGATCTCATGCAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K104224-0022-596-B04C-NJ50
GCTTAGGCTCCCATGTTTCGCAACTTGCAAGAGTTGAATATTATTGTTTTTGAAAAGGAA
TAAGAGAGCCTTCTTGCATATTCCCATTACTGACTCCCAAAGACCTCGCAATCTGTTGCA
TTTCACAAGCGTATTCAGCAGCATCTATACCAATTCTCAGATCATGTTCAAGAACACCTG
CTCTATTCAAATCTACTTGCACCATTAGAGTAACCAACTAATACATAAGAAACACAAACA
TCTTGCAGCTGAAGCCTAAAACCAATACAAGAATCTGATAAAACCAAACTGAAACTTGTC
ATAAATTCCATATGAACCTGAGAGTAATCTCCTGTGTCTGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCC
   
Sequence Name 40-K104225-0022-596-B04C-OriL
GenBank Accession LN503695 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K104225-0022-596-B04C-OriL
CATATGGAATTATGACAAGTTTCAGTTTGGTTTTATCAGATTCTTGTATTGGTTTTAGGC
TTCAGCTGCAAGATGTTTGTGTTTCTTATGTATTAGTTGGTTACTCTAATGGTGCAAGTA
GATTTGAATAGAGCAGGTGTTCTTGAACATGATCTGAGAATTGGTATAGATGCTGCTGAA
TACGCTTGTGAAATGCAACAGATTGCGAGGTCTTTGGGAGTCAGTAATGGGAATATGCAA
GAAGGCTCTCTTATTCCTTTTCAAAAACAATAATATTCAACTCTTGCAAGTTGCGAAACA
TGGGAGCCTAAGCGTGTAAGAATAACCCACTGAAACTCATATATTGCATGAGAT

Last Updated on 06.12.2018 16:11