Sequences from confirmation amplicon


Confirmation sequences for line 602B10 and hit F11C1 are shown.
Line ID 602B10
Locus F11C1 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18669174
Primers used for PCR 04jq - 8474, show primer details
   
Sequence Name 75-K113079-0022-602-B10C-oriL
GenBank Accession LN513272 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K113079-0022-602-B10C-oriL
CTTCATATATCTCTTACAAAAATCATATGTGGGTTGGGACAAAATGGACCCTACAAATGT
CACAAAACTCAGCTAACAATTAAACATCTAACTTGTAAATGTTCCTAAAGCTTCACACCC
AGACAACAAATACTCTTCACCTTATGTGAACATTTCATTAACTCTTACCCATACCTAAGT
CCAACCTTTCACATCGTATATAGTATATAGTTCATGTATCGACGAAAATTTGGTCAATTT
TAATAAATATAATGAGTACCTACAGATCTGATCCAGCTTCTTTTCTGCTCCATTTTTTTA
AACGATTACGAGATCTAAGGTTTAAATCCATATTCCTAAAAACTACTGTACGTTGAAGGA
TTCCATTAAATAAATAAATAGGAAAAAGGGGTCGA
   
Sequence Name 69-K113078-0022-602-B10C-04jq
GenBank Accession LN512240 [GenBank]
Sequencing primer gene specific primer, 04jq: 5'-TCGACCCCTTTTTCCTATTTATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K113078-0022-602-B10C-04jq
TTTTAGGATATGGATTTAACCTTAGATCTCGTAATCGTTTAAAAAATGGAGCAGAAAAGA
AGCTGGATCAGATCTGTAGGTACTCATTATATTTATTAAAATTGACCAAATTTTCGTCGA
TACATGAACTATATACTATATACGATGTGAAAGGTTGGACTTAGGTATGGGTAAGAGTTA
ATGAAATGTTCACATAAGGTGAAGAGTATTTGTTGTCTGGGTGTGAAGCTTTAGGAACAT
TTACAAGTTAGATGTTTAATTGTTAGCTGAGTTTTGTGACATTTGTAGGGTCCATTTTGT
CCCAACCACATATGATTTTTGTAAGAGATATATGGAAGACCATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTA

Last Updated on 06.12.2018 16:11