Sequences from confirmation amplicon


Confirmation sequences for line 604C03 and hit At5g03720 are shown.
Line ID 604C03
Locus At5g03720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:972547
Primers used for PCR TA38 - 8474, show primer details
   
Sequence Name 32-K030141-022-604-C03C-8409
GenBank Accession LN500456 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K030141-022-604-C03C-8409
CATTTACAATTGAAATCTTTTGGTTTCATCATTTTTCTTCGTTTCCTACCGTGTCCACAT
GTCGAGCTGTGCCTCTGCTTTGCTGCTGAAGCTCAACCATTTCCTCCATCAATGCACGCC
GCTCTTTCCTCAGCTTCTCAATCTCTCCTCCAACCTCAGTAGGTGACCCTTGGCTTTGGC
TAGTGCTACTGCAGCAAGTTTGGTTGGATTGTGGTGATCGACGACGATGAATGTTCTTCA
GAAGATGCTTCTTGCCTCTAAGGAAAGCCTCGTTAGCGAATTCCCACTTGTCAGTATCAA
TCTTTCGAAATCCCTGGAAACATTCCAACCCCAAAAAAACAACTCAAACAAGCTCAAGAT
AAAGTAAGTAAACACATTTACATCAAGACAAGACCAAATATCTCAAGATATTAGAAAGAT
TCTACCTCTGAAGTGTGAAGAACATGTGAGTAATGATGTCTTAAACCAGACTGAAGTTAA
CATTGTGAGAAAATAACAAAACTTCATAAACCAAAATTCTCCATTCAAACGAAACCCATA
   
Sequence Name 32-K030141-022-604-C03C-TA38
GenBank Accession LN500457 [GenBank]
Sequencing primer gene specific primer, TA38: 5'-ACTTATGGTTTCGTTTGAATGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K030141-022-604-C03C-TA38
TATTTTCTCACAATGTTAACTTCAGNTCTCGGTTTAAGACATCATTACTCACACTGTTCT
TCACACTTCAGAGGTAGAATCTTTCTAATATCTTGAGATATTTGGTCTTGTCTTGATGTA
AATGTGTTTACTTACTTTATCTTGAGCTTGTTTGAGTTGTTTTTTTGGGGTTGGAATGTT
TCCAGGGATTTCGAAAGATTGATACTGACAAGTGGGAATTCGCTAACGAGGCTTTCCTTA
GAGGCAAGAAGCATCTTCTGAAGAACATTCATCGTCGTCGATCACCACAATCCAACCAAA
CTTGCTGCAGTAGCACTAGCCAAAGCCAAGGGTCACCTACTGAGGTTGGAGGAGAGATTG
AGAAGCTGAGGAAAGAGCGGCGTGCATTGATGGAGGAAATGGTTGAGCTTCAGCAGCAAA
GCAGAGGCACAGCTCGACATGTGGACACGGTAGGAAACGAAGAAAAATGATGAAACCAAA
AGATTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACT

Last Updated on 06.12.2018 16:11