Sequences from confirmation amplicon


Confirmation sequences for line 621B04 and hit At3g09960 are shown.
Line ID 621B04
Locus At3g09960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3062902
Primers used for PCR PU02 - 8474, show primer details
   
Sequence Name 09-K024285-022-621-B04C-PU02
GenBank Accession LN490007 [GenBank]
Sequencing primer gene specific primer, PU02: 5'-CTCCATTGATTCTTCTTTCTGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K024285-022-621-B04C-PU02
ATAGTTTATGATTAGACGTTTGTCTTTGTCGGAAACTGTAAATATAGGTCAAGTGATCGA
TCATGTAATTGGTGATCAGTCAAATAAGTAATGATTGGATCTTTGGTTAACCAAAAATAT
AATTGAATCTTTCTCGATTTGGAGTTTTCATGTTCTCATCTCTGTACTAGTGAAATTATG
AAATTTTATAACTGTTTTGAATCTATTGGTATTGGTTTTGAAACTTGTTTGTTTTCTTTT
CCTTGATAGATTTGATAAAGGCAGTACCTGAGAGTCACAAGAAATTCTTGACCAATATGG
TTTGGGTACATAAAGAGGTAAGGGCTTTTCTCCTACCATGTGACGTTTCTTAGGAACAAA
TGTTCAATTGTAGATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGAC
GTGTCTACATTC
   
Sequence Name 10-K024285-022-621-B04C-8409
GenBank Accession LN490480 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K024285-022-621-B04C-8409
CATGTAGATTTCCCGGACATGAAGCCATCTACAATTGAACATTTGTTCCTAAGAAACGTC
ACATGGTAGGAGAAAAGCCCTTACCTCTTTATGTACCCAAACCATATTGGTCAAGAATTT
CTTGTGACTCTCAGGTACTGCCTTTATCAAATCTATCAAGGAAAAGAAAACAAACAAGTT
TCAAAACCAATACCAATAGATTCAAAACAGTTATAAAATTTCATAATTTCACTAGTACAG
AGATGAGAACATGAAAACTCCAAATCGAGAAAGATTCAATTATATTTTTGGTTAACCAAA
GATCCAATCATTACTTATTTGACTGATCACCAATTACATGATCGATCACTTGACCTATAT
TTACAGTTTCCGACAAAGACAAACGTCTAATCATAAACTATAACCAGAAAGAA

Last Updated on 06.12.2018 16:11