Sequences from confirmation amplicon


Confirmation sequences for line 622A04 and hit At4g15280 are shown.
Line ID 622A04
Locus At4g15280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8719506
Primers used for PCR NG72 - 8474, show primer details
   
Sequence Name 56-K102368-0022-622-A04C-8409
GenBank Accession LN509058 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K102368-0022-622-A04C-8409
TTACAATTGAATATATCCTGGACAAGAAAGCTCGCGGGATTCGTGGTGGACATGTTCTGT
TCCTCGATGATCGATGTAGCTAACGAGTTTGGAGTTCCGTGTTATATGGTATACACATCG
AACGCTACGTTTTTAGGAACCATGCTTCACGTTCAACAAATGTACGATCAAAAGAAGTAT
GACGTCAGCGAGTTAGAAAACTCGGTCACCGAGTTGGAGTTTCCGTCTCTGACTCGTCCT
TATCCAGTGAAGTGTCTTCCTCATATCCTCACTTCAAAGGAGTGGTTACCTCTCTCTCTA
GCTCAAGCTAGGTGTTTCCGGAAGATGAAGGGTATTTTGGTAAATACAGTTGCTGAGCTT
GAACCTCACGCTTTGAAAATGTTCAATATTAATGGTGACGATCTTCCTCAAGTTTATCCT
GTTGGACCAGTGTTGCATCTCGAAAACGGCAATGACGATGATGAGAAGCAATCGGAAATT
TTGCGGTGGCTCGACGAGCAACCGTCTAAATCTGTTGTGTTTCTCTGCTTTGGGAGCTTG
GGAGGTTTCACTGAAGAACAAACAAGAGAAACCGCTGTGGCCCTAGATAGAAGCGGTCAG
CGGTTTCTTTGGTGTCTTCGTCACGCATCGCCNAATATAAAAACAGATC
   
Sequence Name 56-K102369-0022-622-A04C-NG72
GenBank Accession LN509059 [GenBank]
Sequencing primer gene specific primer, NG72: 5'-CGATCTGTTTTTATATTTGGCGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K102369-0022-622-A04C-NG72
CGCTGACGCTTCATCTAGGGCCCAGCGGTTTCTCTTGTTTGTTCTTCAGTGAAACCTCCC
AAGCTCCCAAAGCAGAGAAACACAACAGATTTAGACGGTTGCTCGTCGAGCCACCGCAAA
ATTTCCGATTGCTTCTCATCATCGTCATTGCCGTTTTCGAGATGCAACACTGGTCCAACA
GGATAAACTTGAGGAAGATCGTCACCATTAATATTGAACATTTTCAAAGCGTGAGGTTCA
AGCTCAGCAACTGTATTTACCAAAATACCCTTCATCTTCCGGAAACACCTAGCTTGAGCT
AGAGAGAGAGGTAACCACTCCTTTGAAGTGAGGATATGAGGAAGACACTTCACTGGATAA
GGACGAGTCAGAGACGGAAACTCCAACTCGGTGACCGAGTTTTCTAACTCGCTGACGTCA
TACTTCTTTTGATCGTACATTTGTTGAACGTGAAGCATGGTTCCTAAAAACGTAGCGTTC
GATGTGTATACCATATAACACGGAACTCCAAACTCGTTAGCTACATCGATCATCGAGGAA
CAGAACATGTCCACCACGAATCCCGCGAGCTTTCTTGTCCAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA

Last Updated on 06.12.2018 16:11