Sequences from confirmation amplicon


Confirmation sequences for line 628D03 and hit At5g07100 are shown.
Line ID 628D03
Locus At5g07100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2204553
Primers used for PCR IS58 - 8474, show primer details
   
Sequence Name 59-K023621-022-628-D03C-8409
GenBank Accession LN509872 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K023621-022-628-D03C-8409
AATTTCCCGGACATGAAGCCATTTCCCTTCTTCTTCTCTCATATCATCTTCTTCTTTGAT
CTTGAAATCACAGATTTTGCCGTCTCCTACGACAGGCACATTTCCAGCGCAATCTCTGAA
CTATAACAATAACGGTTTGCTCATTGACAAAAATGAAATCAAATATGAAGACACAACTCC
TCCCTTGTTCCTACCATCTGTAGGATCCATTTTTGCATTTTTAGCTTCTGCTTTATTATC
TCTTAAGACATTCTAGTTTTAAACTATCTCATTTTCAGATGGTAACTCAGCCTTTACCTC
AACTGGATTTATTCAAATCCGAAATCATGTCGAGTAACAAAACCTCTGATGACGGCTACA
ATTGGCGCAAATACGGGCAGAAGCAAGTCAAAGGAAGCGAAAACCCGAGGAGTTACTTCA
AATGCACGTATCCAAATTGTCTCACAAAGAAGAAAGTAGAGACGTCTCTTGTGAAGGGTC
AGATGATTGAGATTGTCTATAAAG
   
Sequence Name 60-K023621-022-628-D03C-IS58
GenBank Accession LN510140 [GenBank]
Sequencing primer gene specific primer, IS58: 5'-TGATTGTGGCTTCCTTTATAGACA-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K023621-022-628-D03C-is58
ATCTCAATCATCTGACCCTTCACAAGAGACGTCTCTACTTTCTTCTTTGTGAGACAATTT
GGATACGTGCATTTGAAGTAACTCCTCGGGTTTTCGCTTCCTTTGACTTGCTTCTGCCCG
TATTTGCGCCAATTGTAGCCGTCATCAGAGGTTTTGTTACTCGACATGATTTCGGATTTG
AATAAATCCAGTTGAGGTAAAGGCTGAGTTACCATCTGAAAATGAGATAGTTTAAAACTA
GAATGTCTTAAGAGATAATAAAGCAGAAGCTAAAAATGCAAAAATGGATCCTACAGATGG
TAGGAACAAGGGAGGAGTTGTGTCTTCATATTTGATTTCATTTTTGTCAATGAGCAAACC
GTTATTGTTATAGTTCAGAGATTGCGCTGGAAATGTGCCTGTCGTAGGAGACGGCAAAAT
CTGTGATTTCAAGATCAAAGAAGAAGATGATATGAGAGAAGAAGAAGGGAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT

Last Updated on 06.12.2018 16:11