Sequences from confirmation amplicon


Confirmation sequences for line 631A04 and hit At4g29210 are shown.
Line ID 631A04
Locus At4g29210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14401793
Primers used for PCR NG65 - 8474, show primer details
   
Sequence Name 63-K102368-0022-631-A04C-8409
GenBank Accession LN510952 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K102368-0022-631-A04C-8409
TTACATTGACGGAGACGGCCATACCGTTTCAAGCCTCGTAAAGTCCAGCTATCTCTCCCG
GAACTCCCATAGACAATGCGCCCAACGATTTCGCACTAGCATCGTTCTTATACATATCCT
GAATCATCAATTCACAAGCTAAACACATTACACACTGAACAACTGGATCAAGAGAGATTA
GTTAGAGAAGCTCTTCTTTTACCTTAAAGCAGCTAATGGAGCAGTTTCTCTCATGTCAAA
GCCTCAGCTTTGGAATCTTTCTGCGAGCTAACAATCAGAAAGAACCCACCGATTCCACTA
GACATTGGATTAACAACACCAACACATAAGT
   
Sequence Name 63-K102369-0022-631-A04C-NG65
GenBank Accession LN510953 [GenBank]
Sequencing primer gene specific primer, NG65: 5'-GCCGTGGCTATTACTTTATGTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K102369-0022-631-A04C-NG65
ATCGGTGGTGGTTCTTTCTTGATTGTTAGCTCGCAGAAAGATTCCAAAGCTGAGGCTTTT
GACATGAGAGAAACTGCTCCATTAGCTGCTTCTAAGGTAAAAGAAGAGCTTCTCTAACTA
ATCTCTCTTGATCCAGTTGTTCAGTGTGTAATGTGTTTAGCTTGTGAATTGATGATTCAG
GATATGTATAAGAACGATGCTAGTGCGAAATCGTTGGGCGCATTGTCTATGGGAGTTCCG
GGAGAGATAGCTGGACTTTACGAGGCTTGGAAACGGTATGGCCGTCTTCCGTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATAGTCAATA

Last Updated on 06.12.2018 16:11