Sequences from confirmation amplicon


Confirmation sequences for line 634B01 and hit At5g11510 are shown.
Line ID 634B01
Locus At5g11510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3680927
Primers used for PCR RS20 - 8474, show primer details
   
Sequence Name 15-K023618-022-634-B01C-8409
GenBank Accession LN492787 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K023618-022-634-B01C-8409
GATTTCCCGGACATGAAGCCATTTACAATTCTCAGGATGACATTCTTTCCTTTCTATATT
CATTTACTGAAGTGAACTGTAATCTTCCTTGTCTAAATGCATGTCACATCTTTTCTTAAC
TCTCAGCTGAATACTTCAAGGATCGAACTGATGTCCAGTGCCTCCACAGGTGGCAAAAGG
TCCTTAATCCTGAACTTGTTAAAGGGCCCTGGACGAAGGAGGTGACATAGTTTTTTCCTC
CACTATTTCTATAACATGTGCATCTTGCACTGTTTGCTTTGAATTTGCAAACATCTCTGA
TGACTGTTTATTGCTCTCGTTTGACATGTGTGTAGGAAGATGAAATGATCGTTCAGTTAA
TCGAAAAATATGGGCCGAAGAAGTGGTCCACTATTGCTCGATTTTTACCTGGCCGTATTG
GAAAGCAATGTAGAGAGAGGTATGCTTATAGAGACAGCTATTTCTCTAACAACAGTGTTT
TACTAGCACTATATAGTTTTTGTCCTCTTTTCAAGATTTTAACTGTGAAGGCTGTTACT
   
Sequence Name 16-K023618-022-634-B01C-RS20
GenBank Accession LN493257 [GenBank]
Sequencing primer gene specific primer, RS20: 5'-GCCACCTATATCAGTAACAGCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K023618-022-634-B01C-RS20
CACAGATTAAAATCTTGAAAAGAGGACAAAAACTATATAGTGCTAGTAAAACACTGTTGT
TAGAGAAATAGCTGTCTCTATAAGCATACCTCTCTCTACATTGCTTTCCAATACGGCCAG
GTAAAAATCGAGCAATAGTGGACCACTTCTTCGGCCCATATTTTTCGATTAACTGAACGA
TCATTTCATCTTCCTACACACATGTCAAACGAGAGCAATAAACAGTCATCAGAGATGTTT
GCAAATTCAAAGCAAACAGTGCAAGATGCACATGTTATAGAAATAGTGGAGGAAAAAACT
ATGTCACCTCCTTCGTCCAGGGCCCTTTAACAAGTTCAGGATTAAGGACCTTTTGCCACC
TGTGGAGGCACTGGACATCAGTTCGATCCTTGAAGTATTCAGCTGAGAGTTAAGAAAAGA
TGTGACATGCATTTAGACAAGGAAGATTACAGTTCACTTCAGTAAATGAATATAGAAAGG
AAAGAATGTCATCCTGAGAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTAC
Primers used for PCR RS19 - 8474, show primer details
   
Sequence Name 61-K023621-022-634-B01C-8409
GenBank Accession LN510409 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K023621-022-634-B01C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAGAATATGTTTGGTTAAAA
TGATTTAGCAAATATGCAGATCATAGAATGTAAAAATGCATATATCCAAATGTCTACTTC
TTGTATCTCTCATGATCAATGATATCATGCAAATGAACAAACACAGGGCAGAGTTTTGTT
GCATATATCAAGAACGTGAGACCAGATTAATTGGCTACATGAACATAGGATTGGAAAGAA
ACTTATAAGAAGAAGCTGATGGAAACATAAATTTCACCTCCTCAGCTGTCCATTGGCCTC
GAGTTGAACGTCTTGCAGGGCCACTAGTCCTCCTGTAAAATCCACACAATTGGTCATGCA
ACTCAATTCTAGGTGTGACAAACATGTCAATCTAGTAGGATATTGTGGCAAGCTAACAAT
TTATTAGTTAGTAAGATATGAGAAAAAAAGAAGCATACCCGTGACGCAATTTCGGGATCC
GTTCCTGAGGGGTTGAAGACTCAGCTTCCATAACTAGTCAATGAAAATGCAAAAATAGTC
ACCCCAATTCCCATACGTTGCCTTTCTTCTCTGCAATGTTGTAACTCCAAAACATTATAT
TTCAAAACTACTATTCCCTCCTTTGTACCATCTCACAATCTTCCTAAGATAAATTGTAAA
GAAAAAAAACACTAAGGATTCAGCTCCAAAAAAAGTCGGTCGCTAATCAGGACAAAACAT
ACTCGAAGCTTGAAAATAATTACAATGACTACTAATTGTGAACT
   
Sequence Name 62-K023621-022-634-B01C-RS19
GenBank Accession LN510648 [GenBank]
Sequencing primer gene specific primer, RS19: 5'-TTTTAAAAATGACTGCGAACAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K023621-022-634-B01C-RS20
CAATTAGTAGTCATTGTAATTATTTTCAAGCTTCGAGTATGTTTTGTCCTGATTAGCGAC
CGACTTTTTTTGGAGCTGAATCCTTAGTGTTTTTTTTCTTTACAATTTATCTTAGGAAGA
TTGTGAGATGGTACAAAGGAGGGAATAGTAGTTTTGAAATATAATGTTTTGGAGTTACAA
CATTGCAGAGAAGAAAGGCAACGTATGGGAATTGGGGTGACTATTTTTGCATTTTCATTG
ACTAGTTATGGAAGCTGAGTCTTCAACCCCTCAGGAACGGATCCCGAAATTGCGTCACGG
GTATGCTTCTTTTTTTCTCATATCTTACTAACTAATAAATTGTTAGCTTGCCACAATATC
CTACTAGATTGACATGTTTGTCACACCTAGAATTGAGTTGCATGACCAATTGTGTGGATT
TTACAGGAGGACTAGTGGCCCTGCAAGACGTTCAACTCGAGGCCAATGGACAGCTGAGGA
GGTGAAATTTATGTTTCCATCAGCTTCTTCTTATAAGTTTCTTTCCAATCCTATGTTCAT
GTAGCCAATTAATCTGGTCTCACGTTCTTGATATATGCAACAAAACTCTGCCCTGTGTTT
GTTCATTTGCATGATATCATTGATCATGAGAGATACAAGAAGTAGACATTTGGATATATG
CATTTTTACATTCTATGATCTGCATATTTGCTAAATCATTTTAACCAAACATATTCT

Last Updated on 06.12.2018 16:11