Sequences from confirmation amplicon


Confirmation sequences for line 635C09 and hit At1g72370 are shown.
Line ID 635C09
Locus At1g72370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27242997
Primers used for PCR 08hd - 8474, show primer details
   
Sequence Name 45-K112477-0022-635-C09C-08hd
GenBank Accession LN505579 [GenBank]
Sequencing primer gene specific primer, 08hd: 5'-TTTACAGGGAACCTGAGGAGACTA-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K112477-0022-635-C09C-08hd
GGTGCACTTCCTGCTCCGAATATGGAATGGTAGGTGGAGACCAATGGACCACCGCTCAGA
TCCCTGATGCTGCATGGCCAGGAGAAGGTCAGGCACCAATCTCTGCTGCACCTGCTGCTG
CTTCTTGGAGTGATTCCGCTGGTGAGTTTTTGATAACCATTTGAACCCTATTCTAACAGA
TCTTGATTCTAATGTTATGTTTATTTTGCTGTTCTAGCTGCACCGGCAGATGGTGGATGG
GAAGCTGCCGCGCCACCCTCAGGGGCACCAGCTGCTGGTTGGGAGTAAAGTTGATAGCCT
TTACTCAATGGTTTAATGGTTATAGACATGCTTTTTTGTTACTTTTGGGATTGAAGAGTT
ATCGAACCGTAACTGTTTTTTCTTCTCACTTGTTTCTCTTAAGACCCTTAGATGTTTTGC
ACCTTTTTGTTTGAATCGATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 22-K112476-0022-635-C09C-8409
GenBank Accession LN496179 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K112476-0022-635-C09C-8409
CATTTCATCGATTCAACAAAAGGTGCAAAACATCTAAGGGTCTTAAGAGAAACAAGTGAG
AAGAAAAAACAGTTACGGTTCGATAACTCTTCAATCCCAAAAGTAACAAAAAAGCATGTC
TATAACCATTAAACCATTGAGTAAAGGCTATCAACTTTACTCCCAACCAGCAGCTGGTGC
CCCTGAGGGTGGCGCGGCAGCTTCCCATCCACCATCTGCCGGTGCAGCTAGAACAGCAAA
ATAAACATAACATTAGAATCAAGATCTGTTAGAATAGGGTTCAAATGGTTATCAAAAACT
CACCAGCGGAATCACTCCAAGAAGCAGCAGCAGGTGCAGCAGAGATTGGTGCCTGACCTT
CTCCTGGCCATGCAGCATCAGGGATCTGAGCGGTGGTCCATTGGTCTCCACCTACCATTC
CATATTCTGGAGCAGGAAGTGCACCATATTCTGCCTGAGGGCCAGCTTCATCTTCATCCT
CTGGCTTAGTCTCCTCAGGTTCCCTGTAA

Last Updated on 06.12.2018 16:11